Vespertiliovirus (taxid:1977144)

VIRION

Non-enveloped, rod of filaments of 230-280 nm long and 10-15 nm in diameter.

GENOME

Circular, ssDNA genome (+) of 7.8 to 8.4 kb encoding for 10 to 12 proteins. Replication occurs via dsDNA intermediate and may involve replicative transposition

GENE EXPRESSION

Each gene is transcribed by host cellular machinery, via a specific promoter. Some genes end by a transcription terminator.

ENZYMES

• Integrase/transposase? [ORF3]

REPLICATION

CYTOPLASMIC

1. Viral protein mediates binding to host membrane.
2. The proteins of the capsid perform the injection of the viral DNA through bacterial membranes into cell cytoplasm.
3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
5. Viral genome is integrated into host chromosome
6. Replication would occur by replicative transposition.
7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
8. New genomes are synthesized
9. Assembly of the viral capsid.
10. New virions are secreted from host cell.