Orthoebolavirus (taxid:3044781)

Ebolavirus is a genus of ssRNA- viruses in the family Filoviridae that infect mammals. Ebolavirus infection in humans leads to fatal hemorrhagic fever.

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Filamentous 970 nm long for Ebolavirus. Diameter is about 80nm.

GENOME

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Negative-stranded RNA linear genome, about 18-19 kb in size. Encodes for seven proteins.

GENE EXPRESSION

The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis.
The primary product of the unedited transcript of GP gene yields a smaller non-structural glycoprotein sGP which is efficiently secreted from infected cells. RNA editing allows expression of full-length GP.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Attachment to host receptors through GP glycoprotein like DC-SIGN and DC-SIGNR
  2. Cellular receptor like HAVCR1(TIM1) binds phosphatidyl serine on virion membrane and a signal is transduced into the cell that trigger the macropinocytosis program by apoptotic mimicry.
  3. The virion enters the cell by Macropinocytosis. In some culture cells, GP glycoprotein can be processed by host Cathepsin L and Cathepsin B into 19kDa GP1. But this processing is not happening in all cells or for all ebolavirus.
  4. GP1 interacts with host NPC1, in late macropinosome and promotes Fusion of virus membrane with the vesicle membrane. The ribonucleocapsid is then released into the cytoplasm.
  5. Sequential transcription, viral mRNAs are capped and polyadenylated by polymerase stuttering in the cytoplasm.
  6. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  7. The ribonucleocapsid interacts with the matrix protein, and buds via the host ESCRT complexes from the plasma membrane, releasing the virion .

Host-virus interaction

Inhibition of host Interferon induction

Vp35 protein prevents host interferon induction by Inhibition of host IKBKE by virus

Inhibition of host interferon signaling

Vp24 protein block interferon signaling by preventing STAT1 signaling. May act by binding host karyopherin ? proteins, thereby preventing them from transporting the tyrosine-phosphorylated transcription factor STAT1 to the nucleus. May directly bind to STAT1 to prevent its signaling .

Antigenic subvertion

Small Glycoprotein (sGP) is a secreted truncated version of GP that prevent host antibodies anti-GP to effectively neutralize it .

Inhibition of acquired immune response

Ebolavirus may encode for a superantigen, thereby inhibiting Vbeta t cells

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

62 entries grouped by protein

6 entries

RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase)

Select_allDeselect_all
L_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
L_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
L_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
L_EBOSM
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
L_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
L_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

7 entries

Nucleoprotein (Ebola NP) (eNP) (Nucleocapsid protein) (Protein N)

Select_allDeselect_all
NCAP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
NCAP_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
NCAP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)

Nucleoprotein (Nucleocapsid protein) (Protein N) (Reston NP) (rNP)

NCAP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)

Nucleoprotein (Nucleocapsid protein) (Protein N) (Reston NP) (rNP)

NCAP_EBOSB
Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)

Nucleoprotein (Nucleocapsid protein) (Protein N)

NCAP_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)

Nucleoprotein (Nucleocapsid protein) (Protein N)

NCAP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

11 entries

Envelope glycoprotein (GP1,2) (GP)

Select_allDeselect_all
VGP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VGP_EBOEC
Zaire ebolavirus (strain Eckron-76) (ZEBOV) (Zaire Ebola virus)
VGP_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VGP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VGP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
VGP_EBORS
Reston ebolavirus (strain Siena/Philippine-92) (REBOV) (Reston Ebola virus)
VGP_EBOSB
Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)
VGP_EBOSM
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
VGP_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
VGP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
VGP_TAFVC
Tai Forest ebolavirus (strain Cote d'Ivoire-94) (TAFV) (Cote d'Ivoire Ebola virus)

6 entries

Membrane-associated protein VP24 (Ebola VP24) (eVP24)

Select_allDeselect_all
VP24_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VP24_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VP24_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)

Membrane-associated protein VP24 (Reston VP24) (rVP24)

VP24_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)

Membrane-associated protein VP24 (Reston VP24) (rVP24)

VP24_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)

Membrane-associated protein VP24 (Reston VP24) (rVP24)

VP24_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

5 entries

Transcriptional activator VP30 (EbolaVP30) (eVP30) (Minor nucleoprotein VP30)

Select_allDeselect_all
VP30_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain

Transcriptional activator VP30 (Ebola VP30) (eVP30) (Minor nucleoprotein VP30)

VP30_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP30_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
VP30_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
VP30_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

5 entries

Polymerase cofactor VP35

Select_allDeselect_all
VP35_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain

Polymerase cofactor VP35 (Ebola VP35) (eVP35)

VP35_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP35_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
VP35_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
VP35_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

Polymerase cofactor VP35 (Ebola VP35) (eVP35)

6 entries

Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40)

Select_allDeselect_all
VP40_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VP40_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VP40_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP40_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
VP40_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
VP40_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

11 entries

Pre-small/secreted glycoprotein (pre-sGP)

Select_allDeselect_all
VSGP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VSGP_EBOEC
Zaire ebolavirus (strain Eckron-76) (ZEBOV) (Zaire Ebola virus)
VSGP_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VSGP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VSGP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
VSGP_EBORS
Reston ebolavirus (strain Siena/Philippine-92) (REBOV) (Reston Ebola virus)
VSGP_EBOSB
Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)
VSGP_EBOSM
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
VSGP_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
VSGP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
VSGP_TAFVC
Tai Forest ebolavirus (strain Cote d'Ivoire-94) (TAFV) (Cote d'Ivoire Ebola virus)

5 entries

Super small secreted glycoprotein (SsGP)

Select_allDeselect_all
VSSGP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VSSGP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VSSGP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
VSSGP_EBOSU
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)
VSSGP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
These structures were created with the help of Colabfold by Jason Nomburg, Nathan Price and Jennifer Doudna (ModelArchive).

Bundibugyo virus taxid:565995

Protein ModelArchive
Envelope glycoprotein (Spike glycoprotein) ma-jd-viral-51071
Matrix protein VP40 (Membrane-associated protein VP40) ma-jd-viral-58753
Membrane-associated protein VP24 ma-jd-viral-67372
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-03362
Polymerase cofactor VP35 ma-jd-viral-58517
Second secreted glycoprotein ma-jd-viral-58978
Small secreted glycoprotein (Soluble secreted glycoprotein) ma-jd-viral-58976
Transcriptional activator VP30 (Minor nucleoprotein VP30) ma-jd-viral-31304

Sudan ebolavirus taxid:186540

Protein ModelArchive
Envelope glycoprotein (Spike glycoprotein) (Structural glycoprotein) ma-jd-viral-51072
Matrix protein VP40 (Membrane-associated protein VP40) ma-jd-viral-58749
Membrane-associated protein VP24 ma-jd-viral-67378
Nonstructural soluble secreted glycoprotein (Small secreted glycoprotein) ma-jd-viral-58977
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-03364
Polymerase cofactor VP35 ma-jd-viral-58513
Second secreted glycoprotein ma-jd-viral-58981
Transcriptional activator VP30 (Minor nucleoprotein VP30) ma-jd-viral-31296

Sudan ebolavirus (strain Human/Uganda/Gulu/2000) taxid:386033

Protein ModelArchive
Envelope glycoprotein (GP1,2) (GP) ma-jd-viral-51072
Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40) ma-jd-viral-58749
Membrane-associated protein VP24 (Reston VP24) (rVP24) ma-jd-viral-67378
Nucleoprotein (Nucleocapsid protein) (Protein N) ma-jd-viral-03364
Polymerase cofactor VP35 ma-jd-viral-58513
Pre-small/secreted glycoprotein (pre-sGP) ma-jd-viral-58977
Transcriptional activator VP30 (EbolaVP30) (eVP30) (Minor nucleoprotein VP30) ma-jd-viral-31296

Tai Forest ebolavirus taxid:186541

Protein ModelArchive
Matrix protein VP40 (Membrane-associated protein VP40) ma-jd-viral-58752
Membrane-associated protein VP24 ma-jd-viral-67374
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-03363
Polymerase cofactor VP35 ma-jd-viral-58514
Putative second secreted glycoprotein ma-jd-viral-58980
Soluble secreted glycoprotein ma-jd-viral-58982
Spike glycoprotein ma-jd-viral-51067
Transcriptional activator VP30 (Minor nucleoprotein VP30) ma-jd-viral-31302

Zaire ebolavirus taxid:186538

Protein ModelArchive
GP1,2 (Virion spike glycoprotein) ma-jd-viral-51069
Matrix protein VP40 (Membrane-associated protein VP40) ma-jd-viral-58748
Membrane-associated protein VP24 ma-jd-viral-67373
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-03365
Polymerase cofactor VP35 ma-jd-viral-58515
SGP (Small non-structural, secreted glycoprotein) (Small secreted glycoprotein) ma-jd-viral-58979
Second non-structural secreted glycoprotein (Second secreted glycoprotein)... ma-jd-viral-58984
Transcriptional activator VP30 (Minor nucleoprotein VP30) ma-jd-viral-31303

Zaire ebolavirus (strain Kikwit-95) taxid:128951

Protein ModelArchive
Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40) ma-jd-viral-58748
Pre-small/secreted glycoprotein (pre-sGP) ma-jd-viral-58979
Transcriptional activator VP30 (EbolaVP30) (eVP30) (Minor nucleoprotein VP30) ma-jd-viral-31303

Zaire ebolavirus (strain Mayinga-76) taxid:128952

Protein ModelArchive
Envelope glycoprotein (GP1,2) (GP) ma-jd-viral-51069
Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40) ma-jd-viral-58748
Matrix protein VP40 (Membrane-associated protein VP40) ma-jd-viral-58748
Membrane-associated protein VP24 (Ebola VP24) (eVP24) ma-jd-viral-67373
Nucleoprotein (Ebola NP) (eNP) (Nucleocapsid protein) (Protein N) ma-jd-viral-03365
Polymerase cofactor VP35 ma-jd-viral-58515
Polymerase cofactor VP35 (Ebola VP35) (eVP35) ma-jd-viral-58515
Pre-small/secreted glycoprotein (pre-sGP) ma-jd-viral-58979
SGP ma-jd-viral-58979
Super small secreted glycoprotein (SsGP) ma-jd-viral-58984
Transcriptional activator VP30 (Ebola VP30) (eVP30) (Minor nucleoprotein VP30) ma-jd-viral-31303
Transcriptional activator VP30 (Minor nucleoprotein VP30) ma-jd-viral-31303
Virion spike glycoprotein ma-jd-viral-51069