Totivirus (taxid:11007)

VIRION

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Non enveloped, icosahedral virion composed of a 60 asymmetric dimers of the single capsid protein (CP), about 40 nm in diameter. The capsid has a T=2* icosahedral symmetry.
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GENOME

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Linear dsRNA genome of 4.6-6.7 kb. Contains 2 overlapping ORFs gag and pol, respectively encoding CP and RdRp.
Some isolates contain additional satellite dsRNAs which encode "killer" proteins and are encapsidated separately.

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a mRNA, which is translocated to the cell cytoplasm where it is translated.
Translation is initiated on a unique internal ribosome entry site (IRES) element situated at the 5'-UTR.
In most totiviruses, the plus-strand viral transcript is flanked by a 5'untranslated region (5'-UTR) and a 3'-UTR and directs the translation of a major CP (Gag) and a minor fusion protein CP-RdRP (Gag-Pol) via a -1 ribosomal frameshift.
In other totiviruses (e.g. Hv190SV), RDRP is detected only as a separate, nonfused polypeptide and may be expressed through a termination-reinitiation mechanism.
Cap-snatching in totivirus L-A furnishes its transcript with a cap structure derived from host mRNAs .

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus remains intracellular.
  2. Transcription of the dsRNA genome by viral polymerase occurs inside the virion, so that dsRNA is never exposed to the cytoplasm. This plus-strand transcript is used as template for translation.
  3. The 5' termini of the transcripts are diphosphorylated.
  4. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  5. Mature virions are transmitted to new cell by cytoplasmic exchange, sporogenesis or hyphal anastomosis.

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

6 entries grouped by protein

2 entries

Major capsid protein (Gag protein) (Major coat protein)

Select_allDeselect_all
GAG_SCVLA
Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) reference strain
GAG_SCVLB
Saccharomyces cerevisiae virus L-BC (ScV-L-BC) (ScVLa) reference strain

1 entry

KP4 killer toxin (Fungal toxin KP4) (Killer protein 4)

KP4T_UMV4
Ustilago maydis P4 virus (UmV4) (UmV-P4)

1 entry

KP6 killer toxin (Killer protein 6)

KP6T_UMV6
Ustilago maydis P6 virus (UmV6) (UmV-P6)

2 entries

Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Gag-Pol protein)

Select_allDeselect_all
RDRP_SCVLA
Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) reference strain
RDRP_SCVLB
Saccharomyces cerevisiae virus L-BC (ScV-L-BC) (ScVLa) reference strain
These structures were created with the help of Colabfold by Jason Nomburg, Nathan Price and Jennifer Doudna (ModelArchive).

Gremmeniella abietina RNA virus L2 taxid:279539

Protein ModelArchive
Putative coat protein ma-jd-viral-15402
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19842

Saccharomyces cerevisiae virus L-BC taxid:42478

Protein ModelArchive
Major capsid protein (Gag protein) (Major coat protein) ma-jd-viral-21444
Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Gag-Pol protein) ma-jd-viral-19944

Xanthophyllomyces dendrorhous virus L1A taxid:1167690

Protein ModelArchive
Capsid protein ma-jd-viral-21440
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19943