Alfamovirus

VIRION

bacilliform with a constant diameter of 18 nm. Three genomic and one subgenomic segments are encapsidated in distinct particles, resulting in different types of virion.

GENOME

Segmented, tripartite linear ssRNA(+) genome composed of RNA1, RNA2, RNA3. Each genomic segment has a 3' tRNA-like structure and a 5’cap.

GENE EXPRESSION

Genomic RNA serves as messenger RNAs. RNA1 and RNA2 encode respectively proteins 1a and 2a, both involved in genome replication and internal transcription of sgRNA4 from the minus-strand copy of RNA3. RNA3 and sgRNA4 are translated respectively into movement and capsid proteins.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. Expression of protein 1a and 2a provides replication proteins.
  4. Replication occurs in viral factories made of membrane vesicles derived from the ER (spherules). A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Subgenomic RNA4 is translated producing capsid proteins.
  7. Assembly of new virus particles.
  8. Viral movement protein triggers the formation of tubular structures that mediate virion cell-to-cell transfer via a tubule-guided mechanism.

Matching UniProtKB/Swiss-Prot entries

10 entries grouped by protein (browse by keywords)

All proteins are shown (view only 4 complete proteome entries)

1 entry

Replication protein 1a

1A_AMVLE
Alfalfa mosaic virus (strain 425 / isolate Leiden) reference strain

4 entries

Capsid protein (CP) (Coat protein)

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CAPSD_AMVLE
Alfalfa mosaic virus (strain 425 / isolate Leiden) reference strain
CAPSD_AMVMA
Alfalfa mosaic virus (strain 425 / isolate Madison)
CAPSD_AMVST
Alfalfa mosaic virus (strain Strasbourg)
CAPSD_AMVYS
Alfalfa mosaic virus (strain YSMV)

4 entries

Movement protein (MP) (Protein 3A)

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MVP_AMVLE
Alfalfa mosaic virus (strain 425 / isolate Leiden) reference strain
MVP_AMVMA
Alfalfa mosaic virus (strain 425 / isolate Madison)
MVP_AMVST
Alfalfa mosaic virus (strain Strasbourg)
MVP_AMVYS
Alfalfa mosaic virus (strain YSMV)

1 entry

RNA-directed RNA polymerase 2a (protein 2a) (EC 2.7.7.48)

RDRP_AMVLE
Alfalfa mosaic virus (strain 425 / isolate Leiden) reference strain