Protoparvovirus

VIRION


Non-enveloped, round, T=1 icosahedral symmetry, 18-26 nm in diameter. The capsid consists of 60 copies of CP protein.

GENOME

Linear, ssDNA genome of about 4 to 6 kb in size. Both positive and negative strands are encapsidated, although the percentage of particles encapsidating the positive strand can be lower depending on the host cell. MVM packages predominantly negative-sense single strands, while LuIII encapsidates strands of both polarities with equal efficiency. ORFs for both the structural and non-structural proteins are located on the same DNA strand.
The genome is replicated through rolling-hairpin mechanism.

GENE EXPRESSION

Host proteins transcribe the genomes into mRNAs. Alternative splicing allows expression of both structural and non-structural proteins. h4. REPLICATION

NUCLEAR

  1. Attachement to host receptors initiates clathrin-mediated endocytosis of the virion into the host cell.
  2. The virion penetrates into the cytoplasm via permeabilization of host endosomal membrane.
  3. Microtubular transport of the virion toward the nucleus.
  4. The viral ssDNA genome penetrates into the nucleus.
  5. The ssDNA is converted into dsDNA by cellular proteins.
  6. dsDNA transcription gives rise to viral mRNAs when host cell enters S phase and translated to produce viral proteins.
  7. Replication occurs through rolling-hairpin mechanism, with NS1 endonuclease binding covalently to the 5' genomic end.
  8. Individual ssDNA genomes are excised from replication concatemers by a process called junction resolution.
  9. These newly synthesized ssDNA can either
    a) be converted to dsDNA and serve as a template for transcription/replication
    b) be encapsidated to form new virions that are released by cell lysis.

Host-virus interaction

Apoptosis modulation

Parvovirus infection induces host cell death in cancerous cells. Apoptosis is the major form of cell death. However, necrosis has also been reported during infection of the minute virus of mice and parvovirus H-1 .
H-1PV NS1 protein expression induces apoptosis via caspases activation and DNA damage signaling. Depending on cell type and growth conditions, H-1PV is able to induce apoptosis, necrosis, or cathepsin B-dependent cell death.

Cell-cycle modulation

H-1PV NS1 protein induces G2/M checkpoint arrest.

Matching UniProtKB/Swiss-Prot entries

25 entries grouped by protein (browse by keywords)

All proteins are shown (view only 19 complete proteome entries)

15 entries

Capsid protein VP1 (Coat protein VP1)

Select_allDeselect_all
CAPSD_MUMIP
Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p))) reference strain
CAPSD_FPV
Feline panleukopenia virus (FPV)
CAPSD_FPV19
Feline panleukopenia virus (strain 193) (FPV)
CAPSD_MEVA
Mink enteritis virus (strain Abashiri) (MEV)
CAPSD_MUMIM
Murine minute virus (strain MVMi) (MVM) (Murine parvovirus)
CAPSD_PAVC
Canine parvovirus type 2 (CPV-2)

Capsid protein VP2 (Coat protein VP2)

CAPSD_PAVC7
Canine parvovirus type 2 (strain Dog/United States/780929/-) (CPV-2)
CAPSD_PAVCB
Canine parvovirus type 2 (isolate Dog/United States/CPV-b/1978) (CPV-2)
CAPSD_PAVCD
Canine parvovirustype 2 (isolate Dog/United States/CPV-d/1988) (CPV-2)
CAPSD_PAVCN
Canine parvovirus type 2 (isolate Dog/United States/CPV-N/1978) (CPV-2)
CAPSD_PAVHH
Hamster parvovirus H1
CAPSD_PAVL3
Parvovirus LuIII
CAPSD_PAVP9
Porcine parvovirus (strain 90HS) (PPV)
CAPSD_PAVPK
Porcine parvovirus (strain Kresse) (PPV)
CAPSD_PAVPN
Porcine parvovirus (strain NADL-2) (PPV)

1 entry

Non-structural protein NS1

NS1_MUMIP
Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p))) reference strain

1 entry

Non-structural protein NS2

NS2_MUMIP
Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p))) reference strain

8 entries

Non-capsid protein NS-1 (NCVP1) (Non-structural protein NS1)

Select_allDeselect_all
VNCS_FPV19
Feline panleukopenia virus (strain 193) (FPV)
VNCS_MEVA
Mink enteritis virus (strain Abashiri) (MEV)
VNCS_MUMIM
Murine minute virus (strain MVMi) (MVM) (Murine parvovirus)
VNCS_PAVCN
Canine parvovirus type 2 (isolate Dog/United States/CPV-N/1978) (CPV-2)

Non-capsid protein NS-1 (Non-structural protein NS1)

VNCS_PAVHH
Hamster parvovirus H1
VNCS_PAVL3
Parvovirus LuIII

Non-capsid protein NS-1 (Non-structural protein NS1)

VNCS_PAVPK
Porcine parvovirus (strain Kresse) (PPV)

Non-capsid protein NS-1 (Non-structural protein NS1)

VNCS_PAVPN
Porcine parvovirus (strain NADL-2) (PPV)

Non-capsid protein NS-1 (Non-structural protein NS1)