Ebolavirus

Filamentous 970 nm long for Ebolavirus. Diameter is about 80nm.

GENOME

Negative-stranded RNA linear genome, about 18-19 kb in size. Encodes for seven proteins.

GENE EXPRESSION

The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis.
The primary product of the unedited transcript of GP gene yields a smaller non-structural glycoprotein sGP which is efficiently secreted from infected cells. RNA editing allows expression of full-length GP.

REPLICATION

CYTOPLASMIC

  1. Attachment to host receptors through GP glycoprotein like DC-SIGN and DC-SIGNR
  2. Cellular receptor like HAVCR1 phosphatidyl serine on virion membrane and a signal is transduced into the cell that trigger the macropinocytosis program by apoptotic mimicry.
  3. The virion enters the cell by Macropinocytosis . In some culture cells, GP glycoprotein can be processed by host Cathepsin L and Cathepsin B into 19kDa GP1 . But this processing is not happening in all cells or for all ebolavirus .
  4. GP1 interacts with host NPC1, in late macropinosome and promotes Fusion of virus membrane with the vesicle membrane . The ribonucleocapsid is then released into the cytoplasm.
  5. Sequential transcription, viral mRNAs are capped and polyadenylated by polymerase stuttering in the cytoplasm.
  6. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  7. The ribonucleocapsid interacts with the matrix protein, and buds via the host ESCRT complexes from the plasma membrane , releasing the virion .

Host-virus interaction

Inhibition of host Interferon induction

Vp35 protein prevents host interferon induction by Inhibition of host IKBKE by virus

Inhibition of host interferon signaling

Vp24 protein block interferon signaling by preventing STAT1 signaling. May act by binding host karyopherin ? proteins, thereby preventing them from transporting the tyrosine-phosphorylated transcription factor STAT1 to the nucleus. May directly bind to STAT1 to prevent its signaling .

Antigenic subvertion

Small Glycoprotein (sGP) is a secreted truncated version of GP that prevent host antibodies anti-GP to effectively neutralize it .

Inhibition of acquired immune response

Ebolavirus may encode for a superantigen, thereby inhibiting Vbeta t cells

Matching UniProtKB/Swiss-Prot entries

62 entries grouped by protein (browse by keywords)

All proteins are shown (view only 45 complete proteome entries)

6 entries

RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase)

Select_allDeselect_all
L_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
L_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
L_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
L_EBOSM
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
L_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
L_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

7 entries

Nucleoprotein (Nucleocapsid protein) (Protein N)

Select_allDeselect_all
NCAP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
NCAP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
NCAP_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
NCAP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
NCAP_EBOSB
Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)
NCAP_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
NCAP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

11 entries

Envelope glycoprotein (GP1,2) (GP)

Select_allDeselect_all
VGP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VGP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VGP_EBOEC
Zaire ebolavirus (strain Eckron-76) (ZEBOV) (Zaire Ebola virus)
VGP_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VGP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VGP_EBORS
Reston ebolavirus (strain Siena/Philippine-92) (REBOV) (Reston Ebola virus)
VGP_EBOSB
Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)
VGP_EBOSM
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
VGP_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VGP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
VGP_TAFVC
Tai Forest ebolavirus (strain Cote d'Ivoire-94) (TAFV) (Cote d'Ivoire Ebola virus)

6 entries

Membrane-associated protein VP24

Select_allDeselect_all
VP24_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VP24_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VP24_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VP24_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP24_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VP24_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

5 entries

Minor nucleoprotein VP30 (Transcription activator VP30)

Select_allDeselect_all
VP30_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VP30_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VP30_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP30_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VP30_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

5 entries

Polymerase cofactor VP35

Select_allDeselect_all
VP35_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VP35_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VP35_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP35_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VP35_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

6 entries

Matrix protein VP40 (Membrane-associated protein VP40)

Select_allDeselect_all
VP40_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VP40_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VP40_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VP40_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VP40_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VP40_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

11 entries

Pre-small/secreted glycoprotein (pre-sGP)

Select_allDeselect_all
VSGP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VSGP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VSGP_EBOEC
Zaire ebolavirus (strain Eckron-76) (ZEBOV) (Zaire Ebola virus)
VSGP_EBOG4
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
VSGP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VSGP_EBORS
Reston ebolavirus (strain Siena/Philippine-92) (REBOV) (Reston Ebola virus)
VSGP_EBOSB
Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)
VSGP_EBOSM
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
VSGP_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VSGP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
VSGP_TAFVC
Tai Forest ebolavirus (strain Cote d'Ivoire-94) (TAFV) (Cote d'Ivoire Ebola virus)

5 entries

Super small secreted glycoprotein (SsGP)

Select_allDeselect_all
VSSGP_EBORR
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain
VSSGP_EBOZM
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain
VSSGP_EBORE
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
VSSGP_EBOSU
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
VSSGP_EBOZ5
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)