Orthotospovirus

VIRION

Enveloped, spherical. Diameter from 80 to 120nm.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 8.8kb, M segment about 4.8kb and S segment about 3kb.
Encodes for six proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment, and is terminated by a strong hairpin sequence at the end of each gene. mRNAs are capped by L protein during synthesis using cap snatching , but are not polyadenylated.
S and M segments uses ambisense strategy to encode forsevral proteins: both genomic ans antigenomic RNA are transcribed. The hairpin sequence is a stop polymerase signal which prevents ambisense transcription from producing dsRNA. MRNA1 encodes for a polyprotein which is cleaved by host protease into Gn and Gc proteins.

REPLICATION

CYTOPLASMIC

In insect:
  1. Virus attaches to host receptors though Gn-Gc glycoprotein dimer, and is endocytosed into vesicles in the host cell.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid segments are released in the cytoplasm.
  3. Transcription, viral mRNAs are capped in the cytoplasm.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. The ribonucleocapsids buds at Golgi apparatus, releasing the virion by exocytosis.
In plant:
  1. Virus attaches to host receptors.
  2. Fusion of virus membrane with the cell membrane; ribonucleocapsid segments are released in the cell.
  3. Transcription, viral mRNAs are capped in the cytoplasm.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. The ribonucleocapsids buds from a cellular membrane, releasing the virion.

Host-virus interaction

Inhibition of host innate immunity

TSWV NSs inhibits Dicer-mediated dsRNA processing thereby inhibiting RNAi-mediated immunity to control innate immune signaling.

Matching UniProtKB/Swiss-Prot entries

18 entries grouped by protein (browse by keywords)

All proteins are shown (view only 7 complete proteome entries)

5 entries

Envelopment polyprotein (M polyprotein)

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GP_TSWV1
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain
GP_INSV
Impatiens necrotic spot virus (INSV)
GP_TSWV
Tomato spotted wilt virus (TSWV)
GP_TSWVD
Tomato spotted wilt virus (isolate D) (TSWV)
GP_TSWVR
Tomato spotted wilt virus (strain Regular2A) (TSWV)

1 entry

RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (Replicase) (Transcriptase)

L_TSWV1
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain

2 entries

Movement protein (MVP) (Non-structural movement protein) (NSm)

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MVP_TSWV1
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain
MVP_INSV
Impatiens necrotic spot virus (INSV)

7 entries

Nucleoprotein (Nucleocapsid protein) (Protein N)

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NCAP_TSWV1
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain
NCAP_INSV
Impatiens necrotic spot virus (INSV)
NCAP_INSVN
Impatiens necrotic spot virus (strain NL-07) (INSV)
NCAP_TSWV3
Tomato spotted wilt virus (strain Brazilian Br-03) (TSWV) (Tomato chlorotic spot virus)
NCAP_TSWVH
Tomato spotted wilt virus (strain Hawaiian) (TSWV)
NCAP_TSWVL
Tomato spotted wilt virus (strain Bulgarian L3) (TSWV)
NCAP_TSWVS
Tomato spotted wilt virus (strain SA-05) (TSWV) (Groundnut ringspot virus)

3 entries

Non-structural protein NS-S

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NSS_TSWV1
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain
NSS_INSVN
Impatiens necrotic spot virus (strain NL-07) (INSV)
NSS_TSWVL
Tomato spotted wilt virus (strain Bulgarian L3) (TSWV)