Adenoviridae (taxid:10508)

VIRION

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Non-enveloped capsid with a pseudo T=25 icosahedral symmetry. The capsid diameter is about 90 nm. The capsid shell consists of 720 hexon subunits arranged as 240 trimers and 12 vertex penton capsomers each with a fiber protruding from the surface.
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GENOME

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Monopartite, linear double-stranded DNA of 35-36kb. The genome has terminally redundant sequences which have inverted terminal repetitions (ITR). The terminal protein (TP) is covalently attached to each end of the genome.

GENE EXPRESSION

Transcription is nuclear, in two phases; early (replication), late (virion assembly). All genes are transcribed by host RNA pol II except virus-associated (VA) gene(s) of primate adenoviruses which is transcribed by RNA pol III. Genes transcribed by RNA pol II give rise to multiple mRNAs that are produced by alternative splicing and use of different poly(A) sites.

ENZYMES

REPLICATION

NUCLEUS

  1. Attachment of the viral fiber glycoproteins to host receptors mediates endocytosis of the virus into the host cell and fiber shedding.
  2. Disruption of the endosome by lytic protein VI releases the viral capsid in the cytosol.
  3. Viral penetration into host nucleus.
  4. Transcription of early genes (E genes) by host RNA pol II, replication of the DNA genome by DNA strand displacement in the nucleus.
  5. Transcription of late genes (L genes) by host RNA pol II, mostly encoding for structural proteins.
  6. Assembly of new virions in the nucleus.
  7. Virions are released by lysis of the cell.

Host-virus interaction

Adaptive immune response inhibition

The adenovirus E19 protein downregulates cell-surface expression of MHC I by binding and retains the heavy chain in ER. E19 binds TAP and acts as a tapasin inhibitor, preventing class I/TAP association .

Apoptosis modulation

Soon after infection, the expression of E1A protein drives the cell cycle into S-phase so that the virus can replicate its genome optimally . However, this arrest leads to the accumulation of host p53 protein and subsequently apoptosis. The apoptosis is then inhibited by different viral proteins including E1B 55K, E4 orf6 or the bcl-2 homolog E1B 19K.

Autophagy modulation

Autophagy is triggered after adenoviral infection and may facilitate viral replication and/or cell lysis. The E1B 19K viral protein interacts with host Beclin 1, removes Bcl-2 (a negative regulator of autophagy) from Beclin-1 and thereby promotes autophagy .

Cell-cycle modulation

The expression of E1A protein drives the cell cycle into S-phase so that the virus can replicate its genome optimally .

Innate immune response inhibition

Adenoviruses do not inhibit the cascade leading to production of interferon-beta directly. However, they evade the host innate immunity by targeting a cellular histone modification. Indeed, the E1A viral protein blocks the IFN-induced H2B mono-ubiquitination and thereby associated ISGs expression.

Matching UniProtKB/Swiss-Prot entries

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