Tombusviridae

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Monopartite (except Dianthovirus which is bipartite), linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement protein, and capsid proteins are translated from subgenomic RNAs. viral suppressor of RNA silencing (VSRs) is expressed by leaky scanning of the MVP subgenomic mRNA.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Viruses of the Tombusviridae family encode proteins acting as suppressors of RNA silencing. Examples are tombusvirus P19/P20 , aureusvirus P14 , dianthovirus movement protein , carmovirus capsid protein P38 .

Matching UniProtKB/Swiss-Prot entries

80 entries grouped by protein (browse by keywords)

All proteins are shown (view only 58 complete proteome entries)

17 entries

Capsid protein (Coat protein) (p41)

Select_allDeselect_all
CAPSD_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

Capsid protein (Coat protein) (p38)

CAPSD_CRSVL
Carnation ringspot virus (isolate Lommel) (CRSV) reference strain

Capsid protein (Coat protein)

CAPSD_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

Capsid protein (Coat protein) (p25)

CAPSD_OCSVU
Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

Capsid protein (p48)

CAPSD_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

Capsid protein (Coat protein) (p26)

CAPSD_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

Capsid protein (p40)

CAPSD_RCNMV
Red clover necrotic mosaic virus (RCNMV) reference strain

Capsid protein (Coat protein)

CAPSD_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain
CAPSD_TCV
Turnip crinkle virus (TCV) reference strain

Capsid protein (Coat protein) (p38)

CAPSD_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain

Capsid protein (p30)

CAPSD_AMCV
Artichoke mottled crinkle virus (AMCV)
CAPSD_CARMV
Carnation mottle virus (CarMV)

Capsid protein (Coat protein) (p38)

CAPSD_CNV
Cucumber necrosis virus (CNV)
CAPSD_CRV
Cymbidium ringspot virus (CymRSV)
CAPSD_MNSV
Melon necrotic spot virus (MNSV)

Capsid protein (Coat protein) (p42)

CAPSD_TBSVB
Tomato bushy stunt virus (strain BS-3) (TBSV)
CAPSD_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

Capsid protein (p30)

2 entries

Movement protein (MP)

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MP_CRSVL
Carnation ringspot virus (isolate Lommel) (CRSV) reference strain
MP_RCNMV
Red clover necrotic mosaic virus (RCNMV) reference strain

4 entries

Double gene block protein 1 (DGBp1) (Movement protein P7)

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MP1_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain
MP1_TCV
Turnip crinkle virus (TCV) reference strain

Double gene block protein 1 (DGBp1) (Movement protein P8)

MP1_CARMV
Carnation mottle virus (CarMV)
MP1_MNSV
Melon necrotic spot virus (MNSV)

Double gene block protein 1 (DGBp1) (Movement protein P7A)

3 entries

Double gene block protein 2 (DGBp2) (Movement protein P9)

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MP2_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain
MP2_TCV
Turnip crinkle virus (TCV) reference strain
MP2_MNSV
Melon necrotic spot virus (MNSV)

Double gene block protein 2 (DGBp2) (Movement protein P7B)

2 entries

Movement protein p22 (p21)

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MP22_TBSVK
Tomato bushy stunt virus (strain Ja9) (TBSV)
MP22_TBSVT
Tomato bushy stunt virus (strain type) (TBSV)

1 entry

Putative movement protein p6.6

MP6_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

3 entries

Probable movement protein p8

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MP8_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain
MP8_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain
MP8_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

10 entries

Movement protein (p22)

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MVP_GRVMC
Groundnut rosette virus (strain MC1) (GRV) reference strain

Movement protein

MVP_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

Movement protein (p27)

MVP_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain
MVP_AMCV
Artichoke mottled crinkle virus (AMCV)
MVP_CNV
Cucumber necrosis virus (CNV)
MVP_CRV
Cymbidium ringspot virus (CymRSV)
MVP_TBSV8
Tomato bushy stunt virus (strain B8) (TBSV)
MVP_TBSVA
Tomato bushy stunt virus (strain A23) (TBSV)
MVP_TBSVB
Tomato bushy stunt virus (strain BS-3) (TBSV)
MVP_TBSVJ
Tomato bushy stunt virus (strain Ja6) (TBSV)

1 entry

Long-distance movement protein (ORF3 protein)

ORF3_GRVMC
Groundnut rosette virus (strain MC1) (GRV) reference strain

1 entry

RNA silencing suppressor p14

P14_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

1 entry

Protein p15

P15_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

14 entries

RNA silencing suppressor p19 (19 kDa symptom severity modulator)

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P19_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain
P19_AMCV
Artichoke mottled crinkle virus (AMCV)
P19_CIRV
Carnation Italian ringspot virus (CIRV)
P19_CNV
Cucumber necrosis virus (CNV)
P19_CRV
Cymbidium ringspot virus (CymRSV)
P19_HARV
Havel river virus (HaRV)
P19_PENSV
Pelargonium necrotic spot virus (PeNSV)
P19_PLV
Pear latent virus (PeLV)
P19_TBSV8
Tomato bushy stunt virus (strain B8) (TBSV)
P19_TBSVA
Tomato bushy stunt virus (strain A23) (TBSV)
P19_TBSVB
Tomato bushy stunt virus (strain BS-3) (TBSV)
P19_TBSVJ
Tomato bushy stunt virus (strain Ja6) (TBSV)
P19_TBSVK
Tomato bushy stunt virus (strain Ja9) (TBSV)
P19_TBSVT
Tomato bushy stunt virus (strain type) (TBSV)

1 entry

Uncharacterized protein p31

P31_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

1 entry

Uncharacterized protein p32

P32_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

2 entries

Uncharacterized protein p6

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P6_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain
P6_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

1 entry

Uncharacterized protein p7

P7_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain

1 entry

Uncharacterized protein p8

P8_OCSVU
Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

15 entries

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

Select_allDeselect_all
RDRP_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_CRSVL
Carnation ringspot virus (isolate Lommel) (CRSV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_GRVMC
Groundnut rosette virus (strain MC1) (GRV) reference strain

RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48)

RDRP_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (111 kDa protein) (Protein p111)

RDRP_OCSVU
Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84)

RDRP_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p112)

RDRP_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84)

RDRP_RCNMV
Red clover necrotic mosaic virus (RCNMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain
RDRP_TCV
Turnip crinkle virus (TCV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82)

RDRP_CARMV
Carnation mottle virus (CarMV)

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_CNV
Cucumber necrosis virus (CNV)
RDRP_CRV
Cymbidium ringspot virus (CymRSV)
RDRP_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82)