Inovirus

VIRION

Non-enveloped, rod of filaments of 7nm in diameter and 700 to 2000nm in length. Helical capsid with adsorption proteins on one end.

GENOME

Circular, ssDNA genome (+) of 4.5 to 8kb encoding for 4 to 10 proteins. Replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Each gene is transcribed by host cellular machinery, via a specific promoter. Some genes end by a transcription terminator.




REPLICATION

CYTOPLASMIC

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane to allow and subsequent interaction of g3p with the integral membrane protein TolA (coreceptor).
  2. The proteins of the capsid perform the injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

replication cycle

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane to allow and subsequent interaction of g3p with the integral membrane protein TolA (coreceptor).
  2. The proteins of the capsid mediate the Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

Matching UniProtKB/Swiss-Prot entries

21 entries grouped by protein (browse by keywords)

All proteins are shown (view only 18 complete proteome entries)

3 entries

Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

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CAPSD_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain

Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (M13 procoat) (Major coat protein)

CAPSD_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
CAPSD_BPH75
Thermus phage PH75 (Bacteriophage PH75)

2 entries

Gene 1 protein (G1P)

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G1P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G1P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

2 entries

Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)

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G3P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G3P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

2 entries

Virion export protein (Gene 4 protein) (G4P)

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G4P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G4P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

2 entries

DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)

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G5P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain

DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)

G5P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

2 entries

Head virion protein G6P (Coat protein D) (G6P)

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G6P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G6P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

2 entries

Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)

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G7P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G7P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

2 entries

Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)

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G9P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G9P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)

1 entry

Bactoprenol-linked glucose translocase

GTRA_BPSFX
Shigella phage SfX (Shigella flexneri bacteriophage X) (Bacteriophage SfX)

1 entry

Bactoprenol glucosyl transferase (EC 2.4.1.-)

GTRB_BPSFX
Shigella phage SfX (Shigella flexneri bacteriophage X) (Bacteriophage SfX)

2 entries

Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

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REP_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
REP_BPF1
Enterobacteria phage f1 (Bacteriophage f1)