Bdellomicrovirus

VIRION

Non-enveloped, round, T=1 icosahedral symmetry, about 30 nm in diameter. The capsid consists of 12 pentagonal trumpet-shaped pentomers. The virion is composed of 60 copies each of the F, G, and J proteins, and 12 copies of the H protein. There are 12 spikes which are each composed of 5 G and one H proteins.

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GENOME

Circular, ssDNA(+) genome of 4.5kb. Replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Early and late genes promoters tightly regulates the timing of gene expression, which is crucial for the replication cycle.

REPLICATION

CYTOPLASMIC

  1. Pilus-mediated adsorption of the virus onto host cell
  2. The proteins of the capsid perform Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA I (RF-I).
  4. Early viral genes are transcribed by host RNA polymerase, producing viral replication proteins.
  5. Viral protein A cleaves RF-I(+) DNA strand at the origin of replication and covalently attaches itself to the DNA.
  6. (+)strand replication occurs by rolling circle, which is converted to dsDNA by host polymerase, generating RF-II molecules (amplification of RF-I).
  7. Late viral genes are transcribed by host RNA polymerase.
  8. Procapsid assembly in the cytoplasm.
  9. Viral protein C binds to replication complex, inducing synthesis and packaging of neo-synthesized (+)ssDNA genomes (RF-III) into procapsids.
  10. Procapsids maturation occurs in host cytoplasm
  11. Mature virions are released from the cell by lysis.

Matching UniProtKB/Swiss-Prot entries

11 entries grouped by protein (browse by keywords)

1 entry

Internal scaffolding protein VP3

B_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Protein VP5

C_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Capsid protein VP1 (VP1)

CAPSD_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Minor spike protein H (H protein) (Pilot protein) (Protein VP2) (VP2)

H_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

DNA binding protein VP8

J_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Uncharacterized protein N

N_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Replication-associated protein VP4 (Rep) (VP4) (EC 3.1.21.-) (EC 6.5.1.1)

REP_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Uncharacterized protein W

W_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Uncharacterized protein X

X_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Uncharacterized protein Y

Y_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

1 entry

Uncharacterized protein Z

Z_BPPHM
Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain