Polerovirus

VIRION

Non-enveloped, spherical virion about 23 nm in diameter with T=3 icosahedral symmetry composed of 180 CP proteins, with some percentage containing a readthrough domain (RTD) extension located on the particle’s surface.

GENOME

Monopartite, linear, ssRNA(+) genome of 5.3-5.7 kb in size with a VPg bound at the 5' end. There is no poly(A) tail or tRNA-like structure at the 3' end.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. The polymerase is expressed by a ribosomal frameshifting near the end of ORF1. Rap1 translation initiates about 1500 nt downstream of the 5' end of the gRNA by an unusual internal ribosome entry site (IRES).
The movement protein (MVP) and P3a are expressed by leaky scanning of the subgenomic mRNA. Suppression of termination of the CP stop codon produces CP-RTD (CP extended), the capsid subunit essential for aphid transmission.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA ORF1 and ORF2 are translated to produce the RdRp fusion protein.
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Expression of the 3' coterminal sgRNAs. Traduction of these sgRNAs yields the capsid, CP-RTD and movement proteins.
  7. Virus assembly.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Polerovirus protein P0 acts as a suppressor of RNA silencing by targeting Argonaute 1 for degradation .

Matching UniProtKB/Swiss-Prot entries

28 entries grouped by protein (browse by keywords)

All proteins are shown (view only 23 complete proteome entries)

5 entries

Major capsid protein (Coat protein) (CP)

Select_allDeselect_all
CAPSD_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
CAPSD_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain
CAPSD_PLRV
Potato leafroll virus (PLrV)
CAPSD_PLRVR
Potato leafroll virus (strain Potato/Canada/Rowhani/1979) (PLrV)
CAPSD_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

3 entries

Minor capsid protein P3-RTD (P74) (Readthrough protein) (RT protein)

Select_allDeselect_all
MCAPS_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
MCAPS_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain
MCAPS_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

6 entries

Movement protein (MP) (17 kDa protein)

Select_allDeselect_all
MVP_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
MVP_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain

Movement protein (MP) (20 kDa protein)

MVP_BWYVG
Beet western yellows virus (isolate GB1) (BWYV)

Movement protein (MP) (20 kDa protein)

MVP_PLRV
Potato leafroll virus (PLrV)

Movement protein (MP) (17 kDa protein) (MP17)

MVP_PLRVR
Potato leafroll virus (strain Potato/Canada/Rowhani/1979) (PLrV)
MVP_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

1 entry

Protein ORF3a

ORF3A_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain

3 entries

Suppressor of silencing P0 (28 kDa protein) (Protein ORF0)

Select_allDeselect_all
P0_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
P0_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain

Suppressor of silencing P0 (29 kDa protein) (Protein ORF0)

P0_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

3 entries

Protein P1 (69.7 kDa protein) (Genome-linked protein precursor) (Protein ORF1)

Select_allDeselect_all
P1_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
P1_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain

Protein P1 (66.2 kDa protein) (Genome-linked protein precursor) (Protein ORF1)

P1_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

2 entries

Uncharacterized protein P6 (ORF6 protein)

Select_allDeselect_all
P6_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
P6_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

2 entries

Replication-associated protein (Rap1)

Select_allDeselect_all
RAP1_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
RAP1_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

3 entries

Protein P1-P2

Select_allDeselect_all
RDRP_PLRV1
Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain
RDRP_TYYVF
Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain
RDRP_PLRVW
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)