Dianthovirus

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits). Genome segments are encapsidated separately into different types of particle

GENOME

Segmented, bipartite, linear ssRNA(+) genome of 3.7-4.0 kb (RNA1) and 1.3-1.4 kb (RNA2). Both RNA1 and RNA2 lack a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the viral suppressor of RNA silencing (VSRs) and capsid proteins are translated from subgenomic RNAs. The movement protein is translated from RNA-2.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid protein. The movement protein is expressed from RNA-2.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Matching UniProtKB/Swiss-Prot entries

6 entries grouped by strain (browse by keywords)

3 entries

Carnation ringspot virus (isolate Lommel) (CRSV) reference strain

Select_allDeselect_all
CAPSD_CRSVL Capsid protein (Coat protein)
MP_CRSVL Movement protein (MP)
RDRP_CRSVL RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

3 entries

Red clover necrotic mosaic virus (RCNMV) reference strain

Select_allDeselect_all
CAPSD_RCNMV Capsid protein (Coat protein)
MP_RCNMV Movement protein (MP)
RDRP_RCNMV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)