Phenuiviridae

VIRION

Enveloped, spherical. Diameter from 80 to 120nm. Glycoproteins at the surface of the envelope are arranged on an icosahedral lattice, with T=12 symmetry.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 6.4kb, M segment about 3.2kb and S segment about 1.7kb.
Encodes for six proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment, and is terminated by a strong hairpin sequence at the end of each gene. These are capped by L protein during synthesis using cap snatching , but are not polyadenylated.
S segment uses ambisense strategy to encode for several proteins: both genomic and antigenomic RNA are transcribed. The hairpin sequence is a stop polymerase signal which prevents ambisense transcription from producing dsRNA. M segment encodes for several polyproteins by leaky scanning, which are cleaved by host protease into Nsm-GN, Nsm, NSm',Gn and Gc proteins.

REPLICATION

CYTOPLASMIC

  1. Virus attaches to host receptors though Gn-Gc glycoprotein dimer, and is endocytosed into vesicles in the host cell.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid segments are released in the cytoplasm.
  3. Transcription, viral mRNAs are capped in the cytoplasm.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. The ribonucleocapsids buds at Golgi apparatus, releasing the virion by exocytosis.

Host-virus interaction

Inhibition of host transcription initiation

The Rift valley fever virus NSs protein instead plays a role in the inhibition of the host transcription initiation by acting on host TFIIH.

Matching UniProtKB/Swiss-Prot entries

35 entries grouped by protein (browse by keywords)

All proteins are shown (view only 18 complete proteome entries)

1 entry

Putative envelope glycoprotein (GP) (Protein pc2)

GLYC_RSVT
Rice stripe virus (isolate T) (RSV) reference strain

4 entries

Envelopment polyprotein (M polyprotein)

Select_allDeselect_all
GP_RVFVZ
Rift valley fever virus (strain ZH-548 M12) (RVFV) reference strain
GP_UUKS
Uukuniemi virus (strain S23) (Uuk) reference strain
GP_PTPV
Punta toro phlebovirus
GP_RVFV
Rift valley fever virus (RVFV)

3 entries

RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (Replicase) (Transcriptase)

Select_allDeselect_all
L_RVFVZ
Rift valley fever virus (strain ZH-548 M12) (RVFV) reference strain
L_UUKS
Uukuniemi virus (strain S23) (Uuk) reference strain
L_TOSV
Toscana virus (Tos)

3 entries

Movement protein (Non-structural protein 4) (NS4) (Protein pc4)

Select_allDeselect_all
MVP_RSVT
Rice stripe virus (isolate T) (RSV) reference strain
MVP_MSTV
Maize stripe virus (MStV)
MVP_RSVM
Rice stripe virus (isolate M) (RSV)

9 entries

Nucleoprotein (Nucleocapsid protein) (Protein N)

Select_allDeselect_all
NCAP_RSVT
Rice stripe virus (isolate T) (RSV) reference strain

Nucleoprotein (Coat protein) (CP) (Nucleocapsid protein) (Protein N) (Protein pc3)

NCAP_RVFVZ
Rift valley fever virus (strain ZH-548 M12) (RVFV) reference strain
NCAP_UUKS
Uukuniemi virus (strain S23) (Uuk) reference strain
NCAP_MSTV
Maize stripe virus (MStV)

Nucleoprotein (Coat protein) (CP) (Nucleocapsid protein) (Protein N) (Protein pc3)

NCAP_PTPV
Punta toro phlebovirus
NCAP_RSVM
Rice stripe virus (isolate M) (RSV)

Nucleoprotein (Coat protein) (CP) (Nucleocapsid protein) (Protein N) (Protein pc3)

NCAP_SFSV
Sandfly fever sicilian virus (SFS)
NCAP_TOSV
Toscana virus (Tos)
NCAP_WYHV
Wheat yellow head virus (WYHV)

Nucleoprotein (Coat protein) (CP) (Nucleocapsid protein) (Protein N) (Protein pc3)

3 entries

Major non-capsid protein (NCP) (20.5 kDa protein) (Protein p4) (Stripe disease-specific protein) (Protein S)

Select_allDeselect_all
NCP_RSVT
Rice stripe virus (isolate T) (RSV) reference strain
NCP_MSTV
Maize stripe virus (MStV)

Major non-capsid protein (NCP) (Protein p4) (Stripe disease-specific protein) (Protein S)

NCP_RSVM
Rice stripe virus (isolate M) (RSV)

5 entries

Non-structural protein NS-S

Select_allDeselect_all
NSS_RVFVZ
Rift valley fever virus (strain ZH-548 M12) (RVFV) reference strain

Non-structural protein S (NSs)

NSS_UUKS
Uukuniemi virus (strain S23) (Uuk) reference strain
NSS_PTPV
Punta toro phlebovirus
NSS_SFSV
Sandfly fever sicilian virus (SFS)
NSS_TOSV
Toscana virus (Tos)

1 entry

Protein p2

P2_RSVT
Rice stripe virus (isolate T) (RSV) reference strain

1 entry

RNA-dependent RNA polymerase (EC 2.7.7.48) (Replicase) (Transcriptase)

RDRP_RSVT
Rice stripe virus (isolate T) (RSV) reference strain

1 entry

Non-structural protein NS5

VNS5_MSTV
Maize stripe virus (MStV)

4 entries

Suppressor of RNA silencing p3 (Protein NS3) (Protein p3)

Select_allDeselect_all
VSR_RSVT
Rice stripe virus (isolate T) (RSV) reference strain
VSR_MSTV
Maize stripe virus (MStV)
VSR_RHBVC
Rice hoja blanca virus (strain cr) (RHBV) (Rice hoja blanca virus (strain Costa Rica))
VSR_RSVM
Rice stripe virus (isolate M) (RSV)