Alpha3microvirus

VIRION

Non-enveloped, round, T=1 icosahedral symmetry, about 30 nm in diameter. The capsid consists of 12 pentagonal trumpet-shaped pentomers. The virion is composed of 60 copies each of the F, G, and J proteins, and 12 copies of the H protein. There are 12 spikes which are each composed of 5 G and one H proteins.


GENOME

Circular, ssDNA(+) genome of 4.4 to 6.1kb. replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Early and late genes promoters tightly regulate the timing of gene expression, which is crucial for the replication cycle.

REPLICATION

CYTOPLASMIC

  1. Pilus-mediated adsorption of the virus onto host cell
  2. The proteins of the capsid perform Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA I (RF-I).
  4. Early viral genes are transcribed by host RNA polymerase, producing viral replication proteins.
  5. Viral protein A cleaves RF-I(+) DNA strand at the origin of replication and covalently attaches itself to the DNA.
  6. (+)strand replication occurs by rolling circle, which is converted to dsDNA by host polymerase, generating RF-II molecules (amplification of RF-I).
  7. Late viral genes are transcribed by host RNA polymerase.
  8. Procapsid assembly in the cytoplasm.
  9. Viral protein C binds to replication complex, inducing synthesis and packaging of neo-synthesized (+)ssDNA genomes (RF-III) into procapsids.
  10. Procapsids maturation occurs in host cytoplasm
  11. Mature virions are released from the cell by lysis.

Matching UniProtKB/Swiss-Prot entries

20 entries grouped by strain (browse by keywords)

10 entries

Enterobacteria phage alpha3 (Bacteriophage alpha-3) reference strain

Select_allDeselect_all
G_BPAL3 Major spike protein G (G protein) (GPG)
J_BPAL3 DNA-binding protein J (J protein) (Small core protein)
SCAFD_BPAL3 External scaffolding protein D (Scaffolding protein D) (GPD)
SCAFB_BPAL3 Internal scaffolding protein B (Scaffolding protein B) (GPB)
VGE_BPAL3 Lysis protein (E protein) (GPE)
VGK_BPAL3 K protein
VGA_BPAL3 A protein (GPA)
VGF_BPAL3 Capsid protein (F protein) (GPF)
VGC_BPAL3 C protein
VGH_BPAL3 Minor spike protein (H protein) (Pilot protein)

10 entries

Enterobacteria phage phiK (Bacteriophage phi-K)

Select_allDeselect_all
G_BPPHK Major spike protein G (G protein) (GPG)
J_BPPHK DNA-binding protein J (J protein) (Small core protein)
SCAFB_BPPHK Internal scaffolding protein B (Scaffolding protein B) (GPB)
SCAFD_BPPHK External scaffolding protein D (Scaffolding protein D) (GPD)
VGE_BPPHK Lysis protein (E protein) (GPE)
VGC_BPPHK C protein
VGA_BPPHK A protein (GPA)
VGH_BPPHK Minor spike protein (H protein) (Pilot protein)
VGK_BPPHK K protein
VGF_BPPHK Capsid protein (F protein) (GPF)