Liefievirus

VIRION

Non-enveloped, head-tail structure. The icosahedral head is about 70 nm in diameter. The non-contractile tail is about 220 nm in length.

GENOME

Linear, dsDNA genome of about 42 kb, encoding about 61-64 proteins. Displays 11 bp 3’ cohesive ends.

GENE EXPRESSION

REPLICATION

CYTOPLASMIC

  1. Adsorption: The phage attaches to target cell adhesion receptors through its tail fibers.
  2. Ejection of the viral DNA into host cell cytoplasm by long flexible tail ejection system.
  3. Transcription and translation of early genes.
  4. Replication of genomic DNA.
  5. Transcription and translation of late genes
  6. Assembly of an empty procapsid and packaging of the genome.
  7. Viral tail fiber assembly and viral tail assembly.
  8. Mature virions are released from the cell by lysis.