Sedoreoviridae (taxid:2946186)

VIRION

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Rotavirus maturation schemes

Non enveloped, icosahedral virion with a double capsid structure (except for cypoviruses and dinovernaviruses which have only the equivalent of the inner capsid). The outer capsid has a T=13 icosahedral symmetry, the inner capsid a T=2* icosahedral symmetry.
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GENOME

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Segmented linear dsRNA genome. Contains 10 to 12 segments coding for 10 to 14 proteins. Segments size range from 0.2 to 3.0 kb. Genome total size range from 18.2 to 30.5 kb.

GENE EXPRESSION

The dsRNA genome is never completely uncoated to prevent activation of antiviral state by the cell in response to dsRNA. Viral polymerase synthesizes mRNA from each of the dsRNA segments. These mRNAs are translocated to the cell cytoplasm where they are translated.
Further proteins are produced by leaky scanning and protein processing.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Attachement to host receptors probably mediates endocytosis of virus into host cell.
  2. Particles are partially uncoated in endolysosomes, but not entirely, and penetrate in the cytoplasm.
  3. Early transcription of the dsRNA genome by viral polymerase occurs inside this sub-viral particle (naked core), so that dsRNA is never exposed to the cytoplasm.
  4. Transcription from each of the dsRNA segments produces mRNA templates for translation.
  5. Viral proteins and genomic RNAs aggregates in cytoplasmic viral factories.
  6. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  7. The capsid is assembled on the sub-viral particle.
  8. Mature virions are released presumably following cell death and associated breakdown of host plasma membrane.

Host-virus interaction

Apoptosis modulation

Bluetongue virus mediates host cell apoptosis via caspases activaton (caspases-8, caspases-9, caspase-3, and caspase-7) .

Innate immune response inhibition

Rotaviruses counteract many antiviral pathways in a strain-specific manner :
NSP1 protein induces the proteasome-dependent degradation of IRF3, IRF5, and IRF7 to prevent their induction of IFN .
NSP1 can also induce proteasome-dependent degradation of the ubiquitin ligase complex protein beta-TrCP, resulting in stabilization of IκB and repress NF-κB . .
Rotavirus can prevent STAT1 and STAT2 nuclear translocation .
BTV NS4 might also be responsible for counteracting the antiviral response of the host .

Host gene expression shutoff by virus

Rotavirus NSP3 protein evicts cytoplasmic poly(A) binding protein (PABP) from translation initiation complexes and thus "shuts off the translation of cellular polyadenylated mRNAs"":/by_protein/1579. Inhibition of host poly(A)-binding protein by virus -> Host translation shutoff .

Matching UniProtKB/Swiss-Prot entries

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