Tombusviridae

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Monopartite (except Dianthovirus which is bipartite), linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5’-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement protein, and capsid proteins are translated from subgenomic RNAs. viral suppressor of RNA silencing is expressed by leaky scanning of the MVP subgenomic mRNA.
Cap-independent translation is mediated possibly through the interaction between a 3’-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Viruses of the Tombusviridae family encode proteins acting as suppressors of RNA silencing. Examples are tombusvirus P19/P20 , aureusvirus P14 , dianthovirus movement protein , carmovirus capsid protein P38 .

Matching UniProtKB/Swiss-Prot entries

77 entries grouped by strain (browse by keywords)

All proteins are shown (view only 46 complete proteome entries)

5 entries

Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

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CAPSD_PMVKCapsid protein (Coat protein) (p26)
MP6_PMVKPutative movement protein p6.6
MP8_PMVKProbable movement protein p8
P15_PMVKProtein p15
RDRP_PMVKRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p112)

5 entries

Tobacco necrosis virus (strain A) (TNV) reference strain

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CAPSD_TNVACapsid protein (p30)
MP8_TNVAProbable movement protein p8
P6_TNVAUncharacterized protein p6
P7_TNVAUncharacterized protein p7
RDRP_TNVARNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82)

4 entries

Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

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CAPSD_CARMSCapsid protein (Coat protein) (p38)
MP1_CARMSDouble gene block protein 1 (DGBp1) (Movement protein P7)
MP2_CARMSDouble gene block protein 2 (DGBp2) (Movement protein P9)
RDRP_CARMSProbable RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

4 entries

Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

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CAPSD_MCMVKCapsid protein (Coat protein) (p25)
P31_MCMVKUncharacterized protein p31
P32_MCMVKUncharacterized protein p32
RDRP_MCMVKRNA-directed RNA polymerase (EC 2.7.7.48) (111 kDa protein) (Protein p111)

4 entries

Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

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CAPSD_POLVPCapsid protein (p40)
MVP_POLVPMovement protein (p27)
P14_POLVPRNA silencing suppressor p14
RDRP_POLVPRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84)

4 entries

Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain

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CAPSD_TBSVCCapsid protein (Coat protein) (p41)
MVP_TBSVCMovement protein (p22)
P19_TBSVCRNA silencing suppressor p19 (19 kDa symptom severity modulator)
RDRP_TBSVCRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

3 entries

Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

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CAPSD_OCSVUCapsid protein (p48)
P8_OCSVUUncharacterized protein p8
RDRP_OCSVURNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84)

3 entries

Red clover necrotic mosaic virus (RCNMV) reference strain

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CAPSD_RCNMVCapsid protein (Coat protein)
MP_RCNMVMovement protein (MP)
RDRP_RCNMVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

4 entries

Cucumber necrosis virus (CNV)

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CAPSD_CNVCapsid protein (Coat protein) (p41)
MVP_CNVMovement protein (p22)
P19_CNVRNA silencing suppressor p19 (19 kDa symptom severity modulator)
RDRP_CNVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

4 entries

Cymbidium ringspot virus (CymRSV)

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CAPSD_CRVCapsid protein (Coat protein) (p41)
MVP_CRVMovement protein (p22)
P19_CRVRNA silencing suppressor p19 (19 kDa symptom severity modulator)
RDRP_CRVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

4 entries

Tobacco necrosis virus (strain D) (TNV)

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CAPSD_TNVDCapsid protein (p30)
MP8_TNVDProbable movement protein p8
P6_TNVDUncharacterized protein p6
RDRP_TNVDRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82)

4 entries

Turnip crinkle virus (TCV)

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CAPSD_TCVCapsid protein (Coat protein) (p38)
MP1_TCVDouble gene block protein 1 (DGBp1) (Movement protein P8)
MP2_TCVDouble gene block protein 2 (DGBp2) (Movement protein P9)
RDRP_TCVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

3 entries

Artichoke mottled crinkle virus (AMCV)

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CAPSD_AMCVCapsid protein (Coat protein) (p41)
MVP_AMCVMovement protein (p22)
P19_AMCVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

3 entries

Carnation mottle virus (CarMV)

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CAPSD_CARMVCapsid protein (Coat protein) (p38)
MP1_CARMVDouble gene block protein 1 (DGBp1) (Movement protein P7)
RDRP_CARMVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

3 entries

Carnation ringspot virus (isolate Lommel) (CRSV)

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CAPSD_CRSVLCapsid protein (Coat protein)
MP_CRSVLMovement protein (MP)
RDRP_CRSVLRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

3 entries

Melon necrotic spot virus (MNSV)

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CAPSD_MNSVCapsid protein (Coat protein) (p42)
MP1_MNSVDouble gene block protein 1 (DGBp1) (Movement protein P7A)
MP2_MNSVDouble gene block protein 2 (DGBp2) (Movement protein P7B)

3 entries

Tomato bushy stunt virus (strain BS-3) (TBSV)

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CAPSD_TBSVBCapsid protein (Coat protein) (p41)
MVP_TBSVBMovement protein (p22)
P19_TBSVBRNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain A23) (TBSV)

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MVP_TBSVAMovement protein (p22)
P19_TBSVARNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain B8) (TBSV)

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MVP_TBSV8Movement protein (p22)
P19_TBSV8RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain Ja6) (TBSV)

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MVP_TBSVJMovement protein (p22)
P19_TBSVJRNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain Ja9) (TBSV)

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MP22_TBSVKMovement protein p22 (p21)
P19_TBSVKRNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain type) (TBSV)

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MP22_TBSVTMovement protein p22 (p21)
P19_TBSVTRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Carnation Italian ringspot virus (CIRV)


P19_CIRVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Havel river virus (HaRV)


P19_HARVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pear latent virus (PeLV)


P19_PLVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pelargonium necrotic spot virus (PeNSV)


P19_PENSVRNA silencing suppressor p19 (19 kDa symptom severity modulator)