Totiviridae (taxid:11006)

VIRION

image

Non enveloped, icosahedral virion composed of a single capsid protein (CP), about 40 nm in diameter. The capsid has a T=2* icosahedral symmetry.
image

GENOME

image

Linear dsRNA genome of 4.6-6.7 kb. Contains 2 overlapping ORFs gag and pol, respectively encoding CP and RdRp. Some totiviruses contain a third small 5'-proximal potential ORF.

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a mRNA, which is translocated to the cell cytoplasm where it is translated.
Translation is initiated on a unique internal ribosome entry site (IRES) element situated at the 5'-UTR.
In most totiviruses, the plus-strand viral transcript is flanked by a 5'untranslated region (5'-UTR) and a 3'-UTR and directs the translation of a major CP (Gag) and a minor fusion protein CP-RdRP (Gag-Pol) via a -1 ribosomal frameshift.
In other totiviruses (e.g. Hv190SV), RDRP is detected only as a separate, non-fused polypeptide and may be expressed through a termination-reinitiation mechanism.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus either remains intracellular (totivirus) or attachement to host receptors mediates endocytosis of virus into host cell (Giardiavirus).
  2. Transcription of the dsRNA genome by viral polymerase occurs inside the virion, so that dsRNA is never exposed to the cytoplasm. This plus-strand transcript is used as template for translation.
  3. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  4. Mature virions are transmitted to new cell during cell division, sporogenesis, cell fusion (totivirus) or are released from the host cell (Giardiavirus).

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

13 entries grouped by protein

3 entries

Major capsid protein (Major coat protein)

Select_allDeselect_all
CAPSD_GLVWB
Giardia lamblia virus (isolate Wang) (GLV) reference strain

Major capsid protein (Gag protein) (Major coat protein)

CAPSD_HV19S
Helminthosporium victoriae virus-190S (Hv190SV) reference strain
CAPSD_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

2 entries

Major capsid protein (Gag protein) (Major coat protein)

Select_allDeselect_all
GAG_SCVLA
Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) reference strain
GAG_SCVLB
Saccharomyces cerevisiae virus L-BC (ScV-L-BC) (ScVLa) reference strain

1 entry

KP4 killer toxin (Fungal toxin KP4) (Killer protein 4)

KP4T_UMV4
Ustilago maydis P4 virus (UmV4) (UmV-P4)

1 entry

KP6 killer toxin (Killer protein 6)

KP6T_UMV6
Ustilago maydis P6 virus (UmV6) (UmV-P6)

1 entry

Putative ORF1 protein

ORF1_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

5 entries

Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Gag-Pol protein)

Select_allDeselect_all
RDRP_GLVWB
Giardia lamblia virus (isolate Wang) (GLV) reference strain
RDRP_HV19S
Helminthosporium victoriae virus-190S (Hv190SV) reference strain

Probable RNA-directed RNA polymerase (EC 2.7.7.48)

RDRP_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

Probable RNA-directed RNA polymerase (EC 2.7.7.48)

RDRP_SCVLA
Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) reference strain
RDRP_SCVLB
Saccharomyces cerevisiae virus L-BC (ScV-L-BC) (ScVLa) reference strain
These structures were created with the help of Colabfold by Jason Nomburg, Nathan Price and Jennifer Doudna (ModelArchive).

Armigeres subalbatus virus SaX06-AK20 taxid:556524

Protein ModelArchive
Coat protein ma-jd-viral-55316
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19711

Beauveria bassiana victorivirus NZL/1980 taxid:1485922

Protein ModelArchive
Coat protein ma-jd-viral-15413
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19836

Botryosphaeria dothidea victorivirus 1 taxid:1547580

Protein ModelArchive
Coat protein ma-jd-viral-15419
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19848

Camponotus nipponicus virus taxid:1765754

Protein ModelArchive
Capsid protein ma-jd-viral-32869
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19952

Camponotus yamaokai virus taxid:1608533

Protein ModelArchive
Capsid protein ma-jd-viral-32872
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19951

Chalara elegans RNA Virus 1 taxid:267285

Protein ModelArchive
Coat protein ma-jd-viral-15410

Drosophila melanogaster totivirus SW-2009a taxid:663282

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19707
Structural protein ma-jd-viral-55315

Fusarium poae victorivirus 1 taxid:1849535

Protein ModelArchive
Capsid protein ma-jd-viral-15406
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19831

Giardia lamblia virus (isolate Wang) taxid:649893

Protein ModelArchive
Major capsid protein (Gag protein) (Major coat protein) ma-jd-viral-42162

Golden shiner totivirus taxid:1856030

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19714

Gremmeniella abietina RNA virus L1 taxid:152217

Protein ModelArchive
Putative coat protein ma-jd-viral-15417

Gremmeniella abietina RNA virus L2 taxid:279539

Protein ModelArchive
Putative coat protein ma-jd-viral-15402
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19842

Leishmania RNA virus 1 - 4 taxid:12530

Protein ModelArchive
Major capsid protein ma-jd-viral-15397
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19849

Leishmania RNA virus 2 - 1 taxid:39116

Protein ModelArchive
Major capsid protein ma-jd-viral-15399
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19838

Leishmania aethiopica RNA virus taxid:1497019

Protein ModelArchive
ORF3 ma-jd-viral-15398
Putative capsid protein ma-jd-viral-15400
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19839

Leptopilina boulardi Toti-like virus taxid:1353795

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19527

Magnaporthe oryzae virus 2 taxid:441999

Protein ModelArchive
Coat protein ma-jd-viral-15416
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19832

Magnaporthe oryzae virus 3 taxid:1661396

Protein ModelArchive
Putative coat protein ma-jd-viral-15407
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19827

Maize-associated totivirus 1 taxid:1729670

Protein ModelArchive
Capsid protein ma-jd-viral-32287
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19703

Nigrospora oryzae victorivirus 1 taxid:1765736

Protein ModelArchive
Coat protein ma-jd-viral-15411
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19833

Panax notoginseng virus A taxid:1777016

Protein ModelArchive
Putative coat protein ma-jd-viral-21434
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19929

Penaeid shrimp infectious myonecrosis virus taxid:282786

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19713
Structural protein ma-jd-viral-47016

Penicillium aurantiogriseum totivirus 1 taxid:1755467

Protein ModelArchive
Coat protein ma-jd-viral-15415
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19847

Penicillium digitatum virus 1 taxid:1833938

Protein ModelArchive
Coat protein ma-jd-viral-15409
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19846

Phlebiopsis gigantea mycovirus dsRNA 1 taxid:349681

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-57314

Phomopsis vexans RNA virus taxid:1580605

Protein ModelArchive
Coat protein ma-jd-viral-15420
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19844

Piscine myocarditis virus AL V-708 taxid:912320

Protein ModelArchive
Coat protein ma-jd-viral-52191
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19528
Structural protein ma-jd-viral-65321

Piscine myocarditis-like virus taxid:1798085

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19530
Structural protein ma-jd-viral-29850
Structural protein ma-jd-viral-52190

Red clover powdery mildew-associated totivirus 1 taxid:1714362

Protein ModelArchive
Capsid protein ma-jd-viral-21436
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19928

Red clover powdery mildew-associated totivirus 2 taxid:1714363

Protein ModelArchive
Capsid protein ma-jd-viral-21410
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19945

Red clover powdery mildew-associated totivirus 3 taxid:1714364

Protein ModelArchive
Capsid protein ma-jd-viral-21438
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19930

Red clover powdery mildew-associated totivirus 5 taxid:1714366

Protein ModelArchive
Capsid protein ma-jd-viral-61198
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19933

Red clover powdery mildew-associated totivirus 6 taxid:1714367

Protein ModelArchive
Capsid protein ma-jd-viral-61197
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19936

Red clover powdery mildew-associated totivirus 7 taxid:1714368

Protein ModelArchive
Capsid protein ma-jd-viral-61199
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19939

Rosellinia necatrix victorivirus 1 taxid:1148491

Protein ModelArchive
Putative capsid protein ma-jd-viral-15418
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19830

Saccharomyces cerevisiae virus L-BC taxid:42478

Protein ModelArchive
Major capsid protein (Gag protein) (Major coat protein) ma-jd-viral-21444
Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Gag-Pol protein) ma-jd-viral-19944

Saccharomyces kudriavzevii virus L-A1 taxid:1921825

Protein ModelArchive
Major coat protein ma-jd-viral-21431

Sclerotinia nivalis victorivirus 1 taxid:1859161

Protein ModelArchive
Coat protein ma-jd-viral-15408
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19851

Tianjin totivirus taxid:1163537

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19715

Tolypocladium cylindrosporum virus 1 taxid:939923

Protein ModelArchive
Coat protein ma-jd-viral-15404
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19840

Trichomonas vaginalis virus 1 taxid:674953

Protein ModelArchive
Capsid protein ma-jd-viral-62208
Capsid protein ma-jd-viral-62208
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-32338

Trichomonas vaginalis virus 2 taxid:674954

Protein ModelArchive
Capsid protein ma-jd-viral-62207
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-32339

Ustilaginoidea virens RNA virus 1 taxid:1312445

Protein ModelArchive
Putative coat protein ma-jd-viral-15405
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19843

Ustilaginoidea virens RNA virus 3 taxid:1460374

Protein ModelArchive
Coat protein ma-jd-viral-15414
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19828

Ustilaginoidea virens RNA virus 5 taxid:1756615

Protein ModelArchive
Coat protein ma-jd-viral-15412
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19829

Ustilaginoidea virens RNA virus L taxid:1561171

Protein ModelArchive
Coat protein ma-jd-viral-15424
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19850

Ustilaginoidea virens RNA virus M taxid:1561172

Protein ModelArchive
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19739

Xanthophyllomyces dendrorhous virus L1A taxid:1167690

Protein ModelArchive
Capsid protein ma-jd-viral-21440
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19943