Orthotospovirus (taxid:2560196)

VIRION

image

c

Enveloped, spherical. Diameter from 80 to 120nm.

GENOME

image

Segmented Negative-stranded RNA linear genome, L segment is about 8.8kb, M segment about 4.8kb and S segment about 3kb.
Encodes for six proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment, and is terminated by a strong hairpin sequence at the end of each gene. mRNAs are capped by L protein during synthesis using cap snatching , but are not polyadenylated.
S and M segments uses ambisense strategy to encode forsevral proteins: both genomic ans antigenomic RNA are transcribed. The hairpin sequence is a stop polymerase signal which prevents ambisense transcription from producing dsRNA. MRNA1 encodes for a polyprotein which is cleaved by host protease into Gn and Gc proteins.

ENZYMES

REPLICATION

IN PLANT:CYTOPLASMIC

  1. Virus penetrates into the cell by effraction or plasmodesmata transport.
  2. Cytoplasmic transcription by viral polymerase on the RNA viral RNA template.
  3. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  4. The ribonucleocapsid can penetrate in neighboring plant cells through plasmodesmata transport
  5. Alternatively budding of particles occurs at the cell membrane, releasing new virions.

IN INSECT:CYTOPLASMIC

  1. Attachment of the viral G glycoproteins to host receptors.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid is released into the cytoplasm.
  3. Cytoplasmic transcription by viral polymerase on the RNA viral RNA template.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. Budding of particles occurs at the cell membrane, releasing new virions.

Host-virus interaction

Inhibition of host innate immunity

TSWV NSs inhibits Dicer-mediated dsRNA processing thereby inhibiting RNAi-mediated immunity to control innate immune signaling.

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

18 entries grouped by strain

5 entries

Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain

Select_allDeselect_all
GP_TSWV1 Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...
L_TSWV1 RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (Replicase) ...
MVP_TSWV1 Movement protein (MVP) (Non-structural movement protein) (NSm)
NCAP_TSWV1 Nucleoprotein (Nucleocapsid protein) (Protein N)
NSS_TSWV1 Non-structural protein NS-S

3 entries

Impatiens necrotic spot virus (INSV)

Select_allDeselect_all
GP_INSV Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G1); ...
NCAP_INSV Nucleoprotein (Nucleocapsid protein) (Protein N)
MVP_INSV Movement protein (MVP) (Non-structural movement protein) (NSm)

2 entries

Impatiens necrotic spot virus (strain NL-07) (INSV)

Select_allDeselect_all
NCAP_INSVN Nucleoprotein (Nucleocapsid protein) (Protein N)
NSS_INSVN Non-structural protein NS-S

2 entries

Tomato spotted wilt virus (strain Bulgarian L3) (TSWV)

Select_allDeselect_all
NCAP_TSWVL Nucleoprotein (Nucleocapsid protein) (Protein N)
NSS_TSWVL Non-structural protein NS-S

1 entry

Tomato spotted wilt virus (TSWV)

GP_TSWV Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...

1 entry

Tomato spotted wilt virus (isolate D) (TSWV)

GP_TSWVD Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...

1 entry

Tomato spotted wilt virus (strain Brazilian Br-03) (TSWV) (Tomato chlorotic spot virus)

NCAP_TSWV3 Nucleoprotein (Nucleocapsid protein) (Protein N)

1 entry

Tomato spotted wilt virus (strain Hawaiian) (TSWV)

NCAP_TSWVH Nucleoprotein (Nucleocapsid protein) (Protein N)

1 entry

Tomato spotted wilt virus (strain Regular2A) (TSWV)

GP_TSWVR Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...

1 entry

Tomato spotted wilt virus (strain SA-05) (TSWV) (Groundnut ringspot virus)

NCAP_TSWVS Nucleoprotein (Nucleocapsid protein) (Protein N)