Leishmaniavirus (taxid:39755)

VIRION

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Non enveloped, icosahedral virion composed of a single capsid protein (CP), about 33 nm in diameter. The capsid has a T=2* icosahedral symmetry.
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GENOME

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Linear dsRNA genome of 5.3 kb. The genome has two large, overlapping ORFs on the positive strand respectively encoding CP and RdRp, and a small 5'-proximal potential ORF.

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a mRNA, which is translocated to the cell cytoplasm where it is translated.
Translation is initiated on a unique internal ribosome entry site (IRES) element situated at the 5'-UTR.
L-A virus seems to furnish its transcript with a cap structure derived from host mRNAs by cap snatching . m(7)Gp from the cap donor is transferred to the 5' end of the viral transcript, thus preserving the 5' α-and β-phosphates of the transcript in the triphosphate linkage of the final product. The plus-strand viral transcript directs the translation of the major CP (Gag) and the minor fusion protein CP-RdRP (Gag-Pol) via a -1 ribosomal frameshift.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus remains intracellular.
  2. Transcription of the dsRNA genome by viral polymerase occurs inside the virion, so that dsRNA is never exposed to the cytoplasm. This plus-strand transcript is used as template for translation.
  3. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  4. Mature virions may be transmitted to new cells during cell division.

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

3 entries grouped by protein

1 entry

Major capsid protein (Major coat protein)

CAPSD_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

1 entry

Putative ORF1 protein

ORF1_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

1 entry

Probable RNA-directed RNA polymerase (EC 2.7.7.48)

RDRP_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain
These structures were created with the help of Colabfold by Jason Nomburg, Nathan Price and Jennifer Doudna (ModelArchive).

Leishmania RNA virus 1 - 4 taxid:12530

Protein ModelArchive
Major capsid protein ma-jd-viral-15397
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19849

Leishmania RNA virus 2 - 1 taxid:39116

Protein ModelArchive
Major capsid protein ma-jd-viral-15399
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19838

Leishmania aethiopica RNA virus taxid:1497019

Protein ModelArchive
ORF3 ma-jd-viral-15398
Putative capsid protein ma-jd-viral-15400
RNA-directed RNA polymerase (EC 2.7.7.48) ma-jd-viral-19839