Kanorauviridae

VIRION

Unknown, discovered using viral metagenomics. Presumably on-enveloped, round, presumably T=1 icosahedral symmetry.

GENOME

Monopartite, circular, ssDNA genome of about 2-3 kb.
The genome is replicated through double-stranded intermediates. The replication (Rep) protein initiates and terminates rolling circle replication, the host DNA polymerase being used for DNA replication itself.

GENE EXPRESSION

The two genes are transcribed by host polymerase.

ENZYMES

• Cress-type Endonuclease [Rep]

REPLICATION

NUCLEAR

1. Virus penetrates into the host cell.
2. Uncoating, the viral ssDNA genome penetrates into the nucleus.
3. Viral ssDNA is converted into dsDNA with the participation of cellular factors. dsDNA transcription gives rise to viral mRNAs.
4. Viral mRNAs are translated to produce viral proteins.
5. Replication may be mediated by a Rep protein, and would occur by rolling circle producing ssDNA genomes.
6. These newly synthesized ssDNA can either
   a) be converted to dsDNA and serve as a template for transcription/replication
   b) be encapsidated by capsid protein and form virions released presumably by cell lysis.