Sinsheimervirus (taxid:1910954)

VIRION

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Non-enveloped, round, T=1 icosahedral symmetry, about 30 nm in diameter. The capsid consists of 12 pentagonal trumpet-shaped pentomers. The virion is composed of 60 copies each of the F, G, and J proteins, and 12 copies of the H protein. There are 12 spikes which are each composed of 5 G and one H proteins.
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GENOME

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Circular, ssDNA(+) genome of 4.4 to 6.1kb. replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Early and late genes promoters tightly regulate the timing of gene expression, which is crucial for the replication cycle.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. The viral particle attaches to host cell by binding host lipopolysaccharides.
  2. The proteins of the capsid perform Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA I (RF-I).
  4. Early viral genes are transcribed by host RNA polymerase, producing viral replication proteins.
  5. Viral protein A cleaves RF-I(+) DNA strand at the origin of replication and covalently attaches itself to the DNA.
  6. (+)strand replication occurs by rolling circle, which is converted to dsDNA by host polymerase, generating RF-II molecules (amplification of RF-I).
  7. Late viral genes are transcribed by host RNA polymerase.
  8. Procapsid assembly in the cytoplasm.
  9. Viral protein C binds to replication complex, inducing synthesis and packaging of neo-synthesized (+)ssDNA genomes (RF-III) into procapsids.
  10. Procapsids maturation occurs in host cytoplasm
  11. Mature virions are released from the cell by lysis.

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

20 entries grouped by protein

1 entry

Protein C

C_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

2 entries

Capsid protein F (F protein) (GPF)

Select_allDeselect_all
CAPSD_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain
CAPSD_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

2 entries

Major spike protein G (G protein) (GPG)

Select_allDeselect_all
G_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain
G_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

Minor spike protein H (H protein) (Pilot protein)

H_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

1 entry

DNA-binding protein J (J protein) (Small core protein)

J_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

1 entry

Protein K

K_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

2 entries

Lysis protein (E protein) (GPE)

Select_allDeselect_all
LYS_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

Lysis protein E (Protein E) (GPE)

LYS_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

Replication-associated protein A (EC 3.1.21.-) (EC 6.5.1.1) (RepA)

REPA_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

2 entries

Internal scaffolding protein B (EC 3.4.-.-) (Scaffolding protein B) (GPB)

Select_allDeselect_all
SCAFB_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain
SCAFB_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

2 entries

External scaffolding protein D (Scaffolding protein D) (GPD)

Select_allDeselect_all
SCAFD_BPPHS
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain
SCAFD_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

A' protein (GPA)

VGA_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

C protein

VGC_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

Minor spike protein (H protein) (Pilot protein)

VGH_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

Small core protein (J protein)

VGJ_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain

1 entry

K protein

VGK_BPS13
Enterobacteria phage S13 (Bacteriophage S13) reference strain