Mastrevirus

VIRION

Non-enveloped, about 38 nm in length and 22 nm in diameter (for MSV), twinned (geminate) incomplete T=1 icosahedral symmetry capsid that contains 22 pentameric capsomers made of 110 capsid proteins (CP). Each geminate particle contains only a single circular ssDNA.

GENOME

Monopartite, circular, ssDNA genome (+) genome of about 2.6-2.8 kb. 3' terminus has no poly(A) tract. There are coding regions in both the virion (positive) and complementary (negative) sense strands.
The genome is replicated through double-stranded intermediates. The replication (Rep) protein initiates and terminates rolling circle replication, the host DNA polymerase being used for DNA replication itself. There is a potential stem-loop structure in the long intergenic region (LIR) that includes a conserved nona-nucleotide sequence (TAATATTAC) where ssDNA synthesis is initiated.
The short intergenic region (SIR) contains bidirectional polyadenylation signals.

GENE EXPRESSION

Transcription is bidirectional from the long intergenic region (LIR) which contains two divergent promoters: a V-(virion) and a C-(complementary) sense. The resulting C- and V-transcripts encode four proteins. On the V-sense: the movement and CP. On the C-sense: RepA and Rep (which is expressed as a splice product of RepA and RepB ORFs).

REPLICATION

NUCLEAR

  1. Virus penetrates into the host cell.
  2. Uncoating, the viral ssDNA genome penetrates into the nucleus.
  3. The ssDNA is converted into dsDNA with the participation of cellular factors.
  4. bidirectional dsDNA transcription from the IR promoter produces viral mRNAs and translation of viral proteins.
  5. Replication is initiated by cleavage of the(+)strand by REP, and occurs by rolling circle producing ssDNA genomes.
  6. These newly synthesized ssDNA can either
    a) be converted to dsDNA and serve as a template for transcription/replication
    b) be encapsidated by capsid protein and form virions released by cell lysis
    c) be transported outside the nucleus, to a neighboring cell through plasmodesmata (cell-cell movement) with the help of viral movement proteins.

Host-virus interaction

Cell-cycle modulation

Mastrevirus protein RepA is responsible for inhibiting host retinoblastoma protein and inducing transition from the G1 to S phase in preparation for virus replication since the virus targets differentiated non-dividing cells. .

Matching UniProtKB/Swiss-Prot entries

55 entries grouped by protein (browse by keywords)

14 entries

Capsid protein (Coat protein) (CP)

Select_allDeselect_all
CAPSD_BEYDV
Bean yellow dwarf virus (BeYDV) reference strain
CAPSD_MISV9
Miscanthus streak virus (isolate 91) (MiSV) reference strain
CAPSD_MSVS
Maize streak virus genotype A (isolate South Africa) (MSV) reference strain
CAPSD_SSVN
Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) reference strain
CAPSD_WDVS
Wheat dwarf virus (isolate Sweden) (WDV) reference strain
CAPSD_CSMV
Chloris striate mosaic virus (CSMV)
CAPSD_MSVK
Maize streak virus genotype A (isolate Kenya) (MSV)
CAPSD_MSVN
Maize streak virus genotype A (isolate Nigeria) (MSV)
CAPSD_MSVPA
Maize streak virus genotype E (isolate Pat) (MSV)
CAPSD_MSVRA
Maize streak virus genotype D (isolate Raw) (MSV)
CAPSD_MSVSE
Maize streak virus genotype C (isolate Set) (MSV)
CAPSD_MSVTA
Maize streak virus genotype B (isolate Tas) (MSV)
CAPSD_PASVK
Panicum streak virus (isolate Kenya) (PanSV)
CAPSD_TYDVA
Tobacco yellow dwarf virus (strain Australia) (TYDV)

13 entries

Movement protein (MP)

Select_allDeselect_all
MP_BEYDV
Bean yellow dwarf virus (BeYDV) reference strain
MP_MISV9
Miscanthus streak virus (isolate 91) (MiSV) reference strain

Putative movement protein (MP)

MP_MSVS
Maize streak virus genotype A (isolate South Africa) (MSV) reference strain
MP_SSVN
Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) reference strain
MP_WDVS
Wheat dwarf virus (isolate Sweden) (WDV) reference strain
MP_MSVK
Maize streak virus genotype A (isolate Kenya) (MSV)
MP_MSVN
Maize streak virus genotype A (isolate Nigeria) (MSV)
MP_MSVPA
Maize streak virus genotype E (isolate Pat) (MSV)
MP_MSVRA
Maize streak virus genotype D (isolate Raw) (MSV)
MP_MSVSE
Maize streak virus genotype C (isolate Set) (MSV)
MP_MSVTA
Maize streak virus genotype B (isolate Tas) (MSV)
MP_PASVK
Panicum streak virus (isolate Kenya) (PanSV)
MP_TYDVA
Tobacco yellow dwarf virus (strain Australia) (TYDV)

14 entries

Replication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

Select_allDeselect_all
REP_BEYDV
Bean yellow dwarf virus (BeYDV) reference strain
REP_MISV9
Miscanthus streak virus (isolate 91) (MiSV) reference strain
REP_MSVS
Maize streak virus genotype A (isolate South Africa) (MSV) reference strain
REP_SSVN
Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) reference strain
REP_WDVS
Wheat dwarf virus (isolate Sweden) (WDV) reference strain
REP_CSMV
Chloris striate mosaic virus (CSMV)
REP_MSVK
Maize streak virus genotype A (isolate Kenya) (MSV)
REP_MSVN
Maize streak virus genotype A (isolate Nigeria) (MSV)
REP_MSVPA
Maize streak virus genotype E (isolate Pat) (MSV)
REP_MSVRA
Maize streak virus genotype D (isolate Raw) (MSV)
REP_MSVSE
Maize streak virus genotype C (isolate Set) (MSV)
REP_MSVTA
Maize streak virus genotype B (isolate Tas) (MSV)
REP_PASVK
Panicum streak virus (isolate Kenya) (PanSV)
REP_TYDVA
Tobacco yellow dwarf virus (strain Australia) (TYDV)

14 entries

Replication-associated protein A (RepA) (EC 3.1.21.-)

Select_allDeselect_all
REPA_BEYDV
Bean yellow dwarf virus (BeYDV) reference strain
REPA_MISV9
Miscanthus streak virus (isolate 91) (MiSV) reference strain
REPA_MSVS
Maize streak virus genotype A (isolate South Africa) (MSV) reference strain
REPA_SSVN
Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) reference strain
REPA_WDVS
Wheat dwarf virus (isolate Sweden) (WDV) reference strain
REPA_CSMV
Chloris striate mosaic virus (CSMV)
REPA_MSVK
Maize streak virus genotype A (isolate Kenya) (MSV)
REPA_MSVN
Maize streak virus genotype A (isolate Nigeria) (MSV)
REPA_MSVPA
Maize streak virus genotype E (isolate Pat) (MSV)
REPA_MSVRA
Maize streak virus genotype D (isolate Raw) (MSV)
REPA_MSVSE
Maize streak virus genotype C (isolate Set) (MSV)
REPA_MSVTA
Maize streak virus genotype B (isolate Tas) (MSV)
REPA_PASVK
Panicum streak virus (isolate Kenya) (PanSV)
REPA_TYDVA
Tobacco yellow dwarf virus (strain Australia) (TYDV)