Totiviridae

VIRION

Non enveloped, icosahedral virion composed of a single capsid protein (CP), about 40 nm in diameter. The capsid has a T=2* icosahedral symmetry.


Virion structure at ViperDB

GENOME

Linear dsRNA genome of 4.6-6.7 kb. Contains 2 overlapping ORFs gag and pol, respectively encoding CP and RdRp. Some totiviruses contain a third small 5'-proximal potential ORF.

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a mRNA, which is translocated to the cell cytoplasm where it is translated.
Translation is initiated on a unique internal ribosome entry site (IRES) element situated at the 5'-UTR.
In most totiviruses, the plus-strand viral transcript is flanked by a 5’untranslated region (5'-UTR) and a 3'-UTR and directs the translation of a major CP (Gag) and a minor fusion protein CP-RdRP (Gag-Pol) via a -1 ribosomal frameshift In other totiviruses (e.g. Hv190SV), RDRP is detected only as a separate, non-fused polypeptide and may be expressed through a termination-reinitiation mechanism.

REPLICATION

CYTOPLASMIC

  1. Virus either remains intracellular (totivirus) or attachement to host receptors mediates endocytosis of virus into host cell (Giardiavirus).
  2. Transcription of the dsRNA genome by viral polymerase occurs inside the virion, so that dsRNA is never exposed to the cytoplasm. This plus-strand transcript is used as template for translation.
  3. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  4. Mature virions are transmitted to new cell during cell division, sporogenesis, cell fusion (totivirus) or are released from the host cell (Giardiavirus).

Matching UniProtKB/Swiss-Prot entries

13 entries grouped by protein (browse by keywords)

All proteins are shown (view only 9 complete proteome entries)

3 entries

Major capsid protein (Major coat protein)

Select_allDeselect_all
CAPSD_GLVWB
Giardia lamblia virus (isolate Wang) (GLV) reference strain

Major capsid protein (Gag protein) (Major coat protein)

CAPSD_HV19S
Helminthosporium victoriae virus-190S (Hv190SV) reference strain
CAPSD_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

2 entries

Major capsid protein (Gag protein) (Major coat protein)

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GAG_SCVLA
Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) reference strain
GAG_SCVLB
Saccharomyces cerevisiae virus L-BC (ScV-L-BC) (ScVLa)

1 entry

KP4 killer toxin (Fungal toxin KP4) (Killer protein 4)

KP4T_UMV4
Ustilago maydis P4 virus (UmV4) (UmV-P4)

1 entry

KP6 killer toxin (Killer protein 6)

KP6T_UMV6
Ustilago maydis P6 virus (UmV6) (UmV-P6)

1 entry

Putative ORF1 protein

ORF1_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

5 entries

Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Gag-Pol protein)

Select_allDeselect_all
RDRP_GLVWB
Giardia lamblia virus (isolate Wang) (GLV) reference strain
RDRP_HV19S
Helminthosporium victoriae virus-190S (Hv190SV) reference strain

Probable RNA-directed RNA polymerase (EC 2.7.7.48)

RDRP_LRVLG
Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

Probable RNA-directed RNA polymerase (EC 2.7.7.48)

RDRP_SCVLA
Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) reference strain
RDRP_SCVLB
Saccharomyces cerevisiae virus L-BC (ScV-L-BC) (ScVLa)