Inovirus

VIRION

Non-enveloped, rod of filaments of 7nm in diameter and 700 to 2000nm in length. Helical capsid with adsorption proteins on one end.

GENOME

Circular, ssDNA genome (+) of 4.5 to 8kb encoding for 4 to 10 proteins. Replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Each gene is transcribed by host cellular machinery, via a specific promoter. Some genes end by a transcription terminator.




REPLICATION

CYTOPLASMIC

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane to allow and subsequent interaction of g3p with the integral membrane protein TolA (coreceptor).
  2. The proteins of the capsid perform the injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

replication cycle

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane to allow and subsequent interaction of g3p with the integral membrane protein TolA (coreceptor).
  2. The proteins of the capsid mediate the Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

Matching UniProtKB/Swiss-Prot entries

89 entries grouped by strain (browse by keywords)

All proteins are shown (view only 84 complete proteome entries)

14 entries

Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

Select_allDeselect_all
CAPSD_BPPF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G3P_BPPF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G6P_BPPF1 Head virion protein G6P (Coat protein D) (G6P)
G5P_BPPF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
INTG_BPPF1 Putative integrase
VG071_BPPF1 8.6 kDa protein (ORF 71)
VG083_BPPF1 Uncharacterized protein ORF83 (ORF083)
VG096_BPPF1 10.9 kDa protein (ORF 96)
VG430_BPPF1 Uncharacterized protein ORF430 (ORF430)
VG424_BPPF1 Uncharacterized protein ORF424 (ORF 424)
VG030_BPPF1 3.2 kDa protein (ORF 30)
VG103_BPPF1 11.2 kDa protein (ORF 103)
VG090_BPPF1 10.1 kDa protein (ORF 90)
VG110_BPPF1 12.0 kDa protein (ORF 110)

9 entries

Enterobacteria phage IKe (Bacteriophage IKe) reference strain

Select_allDeselect_all
CAPSD_BPIKE Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G9P_BPIKE Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G5P_BPIKE DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G7P_BPIKE Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G4P_BPIKE Virion export protein (Gene 4 protein) (G4P)
G1P_BPIKE Gene 1 protein (G1P)
G3P_BPIKE Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G6P_BPIKE Head virion protein G6P (Coat protein D) (G6P)
REP_BPIKE Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

9 entries

Enterobacteria phage If1 (Bacteriophage If1) reference strain

Select_allDeselect_all
CAPSD_BPIF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G3P_BPIF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G1P_BPIF1 Gene 1 protein (G1P)
G7P_BPIF1 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G5P_BPIF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G6P_BPIF1 Head virion protein G6P (Coat protein D) (G6P)
G9P_BPIF1 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G4P_BPIF1 Virion export protein (Gene 4 protein) (G4P)
REP_BPIF1 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

9 entries

Enterobacteria phage M13 (Bacteriophage M13) reference strain

Select_allDeselect_all
CAPSD_BPM13 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (M13 procoat) (Major coat protein)
G3P_BPM13 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G6P_BPM13 Head virion protein G6P (Coat protein D) (G6P)
G1P_BPM13 Gene 1 protein (G1P)
G5P_BPM13 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G4P_BPM13 Virion export protein (Gene 4 protein) (G4P)
G9P_BPM13 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G7P_BPM13 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
REP_BPM13 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

9 entries

Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

Select_allDeselect_all
CAPSD_BPPF3 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein) (Pf3 coat protein)
G6P_BPPF3 Head virion protein G6P (Coat protein D) (G6P)
G5P_BPPF3 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G3P_BPPF3 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
VG301_BPPF3 Putative assembly protein ORF301 (ORF301)
VG071_BPPF3 7.9 kDa protein (ORF 71)
VG430_BPPF3 Putative assembly protein ORF430 (ORF430)
REP_BPPF3 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
VG058_BPPF3 6.4 kDa protein (ORF 58)

8 entries

Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain

Select_allDeselect_all
CAPSD_BPI22 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G9P_BPI22 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G1P_BPI22 Gene 1 protein (G1P)
G4P_BPI22 Virion export protein (Gene 4 protein) (G4P)
G3P_BPI22 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G7P_BPI22 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G5P_BPI22 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
REP_BPI22 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

8 entries

Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain

Select_allDeselect_all
CAPSD_BPPHL Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G3P_BPPHL Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G6P_BPPHL Head virion protein G6P (Coat protein D) (G6P)
G7P_BPPHL Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G1P_BPPHL Gene 1 protein (G1P)
G5P_BPPHL DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G9P_BPPHL Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
REP_BPPHL Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

9 entries

Enterobacteria phage f1 (Bacteriophage f1)

Select_allDeselect_all
CAPSD_BPF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G3P_BPF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G7P_BPF1 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G5P_BPF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G6P_BPF1 Head virion protein G6P (Coat protein D) (G6P)
G1P_BPF1 Gene 1 protein (G1P)
G9P_BPF1 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G4P_BPF1 Virion export protein (Gene 4 protein) (G4P)
REP_BPF1 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

9 entries

Enterobacteria phage fd (Bacteriophage fd)

Select_allDeselect_all
CAPSD_BPFD Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G6P_BPFD Head virion protein G6P (Coat protein D) (G6P)
G1P_BPFD Gene 1 protein (G1P)
G7P_BPFD Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G9P_BPFD Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G5P_BPFD DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G4P_BPFD Virion export protein (Gene 4 protein) (G4P)
G3P_BPFD Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
REP_BPFD Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

2 entries

Shigella phage SfX (Shigella flexneri bacteriophage X) (Bacteriophage SfX)

Select_allDeselect_all
GTRB_BPSFX Bactoprenol glucosyl transferase (EC 2.4.1.-)
GTRA_BPSFX Bactoprenol-linked glucose translocase

1 entry

Enterobacteria phage ZJ/2 (Bacteriophage ZJ-2)

CAPSD_BPZJ2 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

1 entry

Thermus phage PH75 (Bacteriophage PH75)

CAPSD_BPH75 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

1 entry

Xanthomonas phage Xf (Bacteriophage Xf)

CAPSD_BPXF Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)