Inovirus

VIRION

Non-enveloped, rod of filaments of 7nm in diameter and 700 to 2000nm in length. Helical capsid with adsorption proteins on one end.

GENOME

Circular, ssDNA genome (+) of 4.5 to 8kb encoding for 4 to 10 proteins. Replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Each gene is transcribed by host cellular machinery, via a specific promoter. Some genes end by a transcription terminator.




REPLICATION

CYTOPLASMIC

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane to allow and subsequent interaction of g3p with the integral membrane protein TolA (coreceptor).
  2. The proteins of the capsid perform the injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

replication cycle

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane to allow and subsequent interaction of g3p with the integral membrane protein TolA (coreceptor).
  2. The proteins of the capsid mediate the Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

Matching UniProtKB/Swiss-Prot entries

30 entries grouped by strain (browse by keywords)

All proteins are shown (view only 26 complete proteome entries)

9 entries

Enterobacteria phage M13 (Bacteriophage M13) reference strain

Select_allDeselect_all
CAPSD_BPM13 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (M13 procoat) (Major coat protein)
G6P_BPM13 Head virion protein G6P (Coat protein D) (G6P)
G1P_BPM13 Gene 1 protein (G1P)
G7P_BPM13 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G4P_BPM13 Virion export protein (Gene 4 protein) (G4P)
G3P_BPM13 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G9P_BPM13 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G5P_BPM13 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
REP_BPM13 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

8 entries

Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain

Select_allDeselect_all
CAPSD_BPPHL Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G9P_BPPHL Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G3P_BPPHL Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G5P_BPPHL DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G6P_BPPHL Head virion protein G6P (Coat protein D) (G6P)
G1P_BPPHL Gene 1 protein (G1P)
G7P_BPPHL Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
REP_BPPHL Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

9 entries

Enterobacteria phage f1 (Bacteriophage f1)

Select_allDeselect_all
CAPSD_BPF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G1P_BPF1 Gene 1 protein (G1P)
G5P_BPF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G7P_BPF1 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G9P_BPF1 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G4P_BPF1 Virion export protein (Gene 4 protein) (G4P)
G6P_BPF1 Head virion protein G6P (Coat protein D) (G6P)
G3P_BPF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
REP_BPF1 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

2 entries

Shigella phage SfX (Shigella flexneri bacteriophage X) (Bacteriophage SfX)

Select_allDeselect_all
GTRA_BPSFX Bactoprenol-linked glucose translocase
GTRB_BPSFX Bactoprenol glucosyl transferase (EC 2.4.1.-)

1 entry

Thermus phage PH75 (Bacteriophage PH75)

CAPSD_BPH75 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

1 entry

Xanthomonas phage Xf (Bacteriophage Xf)

CAPSD_BPXF Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)