Polerovirus

VIRION

Non-enveloped, spherical virion about 23 nm in diameter with T=3 icosahedral symmetry composed of 180 CP proteins, with some percentage containing a readthrough domain (RTD) extension located on the particle’s surface.

GENOME

Monopartite, linear, ssRNA(+) genome of 5.3-5.7 kb in size with a VPg bound at the 5' end. There is no poly(A) tail or tRNA-like structure at the 3' end.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. The polymerase is expressed by a ribosomal frameshifting near the end of ORF1. Rap1 translation initiates about 1500 nt downstream of the 5' end of the gRNA by an unusual internal ribosome entry site (IRES).
The movement protein (MVP) and P3a are expressed by leaky scanning of the subgenomic mRNA. Suppression of termination of the CP stop codon produces CP-RTD (CP extended), the capsid subunit essential for aphid transmission.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA ORF1 and ORF2 are translated to produce the RdRp fusion protein.
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Expression of the 3' coterminal sgRNAs. Traduction of these sgRNAs yields the capsid, CP-RTD and movement proteins.
  7. Virus assembly.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Polerovirus protein P0 acts as a suppressor of RNA silencing by targeting Argonaute 1 for degradation .

Matching UniProtKB/Swiss-Prot entries

23 entries grouped by strain (browse by keywords)

Only complete proteome entries are shown (view all 28 entries)

8 entries

Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) reference strain

Select_allDeselect_all
CAPSD_PLRV1 Major capsid protein (Coat protein) (CP)
MVP_PLRV1 Movement protein (MP) (17 kDa protein)
MCAPS_PLRV1 Minor capsid protein P3-RTD (P74) (Readthrough protein) (RT protein)
P0_PLRV1 Suppressor of silencing P0 (28 kDa protein) (Protein ORF0)
P1_PLRV1 Protein P1 (69.7 kDa protein) (Genome-linked protein precursor) (Protein ORF1) [Cleaved into: Serine ...
P6_PLRV1 Uncharacterized protein P6 (ORF6 protein)
RAP1_PLRV1 Replication-associated protein (Rap1)
RDRP_PLRV1 Protein P1-P2 [Cleaved into: Serine protease (EC 3.4.21.-); RNA-directed RNA polymerase (EC ...

7 entries

Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) reference strain

Select_allDeselect_all
CAPSD_TYYVF Major capsid protein (Coat protein) (CP)
MVP_TYYVF Movement protein (MP) (20 kDa protein)
MCAPS_TYYVF Minor capsid protein P3-RTD (P74) (Readthrough protein) (RT protein)
P0_TYYVF Suppressor of silencing P0 (29 kDa protein) (Protein ORF0)
P1_TYYVF Protein P1 (66.2 kDa protein) (Genome-linked protein precursor) (Protein ORF1) [Cleaved into: Serine ...
ORF3A_TYYVF Protein ORF3a
RDRP_TYYVF Protein P1-P2 [Cleaved into: Serine protease (EC 3.4.21.-); RNA-directed RNA polymerase (EC ...

8 entries

Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

Select_allDeselect_all
CAPSD_PLRVW Major capsid protein (Coat protein) (CP)
MCAPS_PLRVW Minor capsid protein P3-RTD (P74) (Readthrough protein) (RT protein)
MVP_PLRVW Movement protein (MP) (17 kDa protein)
P6_PLRVW Uncharacterized protein P6 (ORF6 protein)
P1_PLRVW Protein P1 (69.7 kDa protein) (Genome-linked protein precursor) (Protein ORF1) [Cleaved into: Serine ...
P0_PLRVW Suppressor of silencing P0 (28 kDa protein) (Protein ORF0)
RDRP_PLRVW Protein P1-P2 [Cleaved into: Serine protease (EC 3.4.21.-); RNA-directed RNA polymerase (EC ...
RAP1_PLRVW Replication-associated protein (Rap1)