Oryzavirus

VIRION

Non enveloped, icosahedral virion with a double capsid structure, about 70 nm in diameter. Pentameric turrets (VP2) sit on the outside of the innermost capsid (VP3). Each turret is surrounded by five peripheral trimers (VP9) and each trimer binds to three clamp proteins (VP4B). The outer incomplete capsid has a T=13 icosahedral symmetry, the inner capsid a T=2* icosahedral symmetry .

GENOME

Segmented linear dsRNA genome. Contains 10 segments coding for 12 proteins. Segments size range from 1162 to 3849 bp. Genome total size is about 26 kb (RRSV).

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a capped mRNA from each dsRNA segment. This capped mRNA is translocated to the cell cytoplasm where it is translated.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Particles are partially uncoated in endolysosomes, but not entirely, and penetrate in the cytoplasm.
  3. Early transcription of the dsRNA genome by viral polymerase occurs inside this sub-viral particle (naked core), so that dsRNA is never exposed to the cytoplasm.
  4. Full-length plus-strand transcripts from each of the dsRNA segments are synthesized. These plus-strand transcripts are used as templates for translation.
  5. Viral proteins and genomic RNAs aggregates in cytoplasmic viral factories.
  6. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  7. The capsid is assembled on the sub-viral particle.
  8. Mature virions are released presumably following cell death and associated breakdown of host plasma membrane.

Host-virus interaction

Suppression of host silencing-mediated antiviral defense

Rice ragged stunt virus (RRSV) Pns6 protein suppresses host cell RNA silencing .

Matching UniProtKB/Swiss-Prot entries

11 entries grouped by strain (browse by keywords)

11 entries

Rice ragged stunt virus (isolate Thailand) (RRSV) reference strain

Select_allDeselect_all
CAPSD_RRSVT Major inner capsid protein VP3 (Major core capsid protein VP3)
VP7_RRSVT Microtubule-associated protein VP7
VP8_RRSVT Structural protein precursor VP8 [Cleaved into: Putative 26 kDa protease; Putative clamp protein ...
VP9_RRSVT Structural protein VP9
VP1_RRSVT Structural protein VP1
VP2_RRSVT Outer capsid protein VP2 [Includes: mRNA guanylyltransferase (EC 2.7.7.50); mRNA ...
VP4B_RRSVT Uncharacterized protein VP4b
VP5_RRSVT Putative minor structural protein VP5
VP10_RRSVT Non-structural protein VP10
MVP_RRSVT Movement and RNA silencing protein VP6
RDRP_RRSVT RNA-directed RNA polymerase (EC 2.7.7.48)