Phytoreovirus

VIRION

Non enveloped, icosahedral, non-turreted virion with a double capsid structure, about 70 nm in diameter. The outer capsid has a T=13 icosahedral symmetry, the inner capsid a T=2* icosahedral symmetry. Each double-layered capsid consists of 260 trimers of the major outer capsid P8 protein and 60 dimers of the inner capsid P3 protein.

GENOME

Segmented linear dsRNA genome. Contains 12 segments coding for 15 proteins. Segment 11 has two in-frame codons resulting in two ORFs. Segment 12 has two additional overlapping ORFs. Segments size range from 1066 to 4423 bp. Genome total size is about 26 kb (WTV).

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a capped mRNA from each dsRNA segment. This capped mRNA is translocated to the cell cytoplasm where it is translated. RDV expresses an additional protein by leaky scanning in the nsp12 gene.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Early transcription of the dsRNA genome by viral polymerase occurs inside this sub-viral particle (naked core), so that dsRNA is never exposed to the cytoplasm.
  3. Full-length plus-strand transcripts from each of the dsRNA segments are synthesized. These plus-strand transcripts are used as templates for translation.
  4. Viral proteins and genomic RNAs aggregates in cytoplasmic viral factories.
  5. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  6. The capsid is assembled on the sub-viral particle.
  7. Mature virions are released presumably following cell death and associated breakdown of host plasma membrane.

Host-virus interaction

Suppression of host silencing-mediated antiviral defense

Rice gall dwarf virus Pns11 and Pns12 proteins as well as rice dwarf virus Pns10 suppress host cell RNA silencing

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Matching UniProtKB/Swiss-Prot entries

56 entries grouped by strain (browse by keywords)

All proteins are shown (view only 25 complete proteome entries)

13 entries

Rice dwarf virus (isolate Fujian) (RDV) reference strain

Select_allDeselect_all
NSP11_RDVF RNA-binding protein (Non-structural protein 11) (Pns11)
NSP12_RDVF Non-structural protein 12A (Pns12A)
NSP4_RDVF Non-structural protein 4 (Pns4)
MCE_RDVF Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase) (EC 2.7.7.50)
MVP_RDVF Movement protein (Non-structural protein 6) (Pns6)
P2_RDVF Minor outer capsid protein P2
P7_RDVF Protein P7 (55 kDa core protein)
OPORF_RDVF Protein OP-ORF
P3_RDVF Outer capsid protein P3 (Core protein P3)
P9_RDVF Minor outer capsid protein P9
P8_RDVF Outer capsid protein P8 (Structural protein P8) [Cleaved into: Outer capsid protein P8'; Small ...
RDRP_RDVF RNA-directed RNA polymerase P1 (EC 2.7.7.48) (Replicase)
VSR_RDVF Suppressor of RNA-mediated gene silencing (Non-structural protein 10) (Pns10)

12 entries

Rice gall dwarf virus (RGDV) reference strain

Select_allDeselect_all
NSP4_RGDV Non-structural protein 4 (Pns4)
NSP12_RGDV Non-structural protein 9 (Pns9)
MCE_RGDV Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase P5) (EC 2.7.7.50)
MVP_RGDV Movement protein (Non-structural protein 7) (Pns7)
NSP11_RGDV RNA-binding protein (Non-structural protein 12) (Pns12)
P2_RGDV Minor outer capsid protein P2
P7_RGDV Protein P7
P9_RGDV Minor outer capsid protein P9 (Protein Pns10)
P3_RGDV Outer capsid protein P3 (Core protein P3)
P8_RGDV Outer capsid protein P8 (Structural protein P8)
RDRP_RGDV RNA-directed RNA polymerase P1 (EC 2.7.7.48) (Replicase)
VSR_RGDV Suppressor of RNA-mediated gene silencing (Non-structural protein 11) (Pns11)

11 entries

Rice dwarf virus (isolate Akita) (RDV)

Select_allDeselect_all
NSP12_RDVA Non-structural protein 12A (Pns12A)
NSP4_RDVA Non-structural protein 4 (Pns4)
NSP11_RDVA RNA-binding protein (Non-structural protein 11) (Pns11)
MCE_RDVA Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase) (EC 2.7.7.50)
MVP_RDVA Movement protein (Non-structural protein 6) (Pns6)
P7_RDVA Protein P7 (55 kDa core protein)
P8_RDVA Outer capsid protein P8 (Structural protein P8) [Cleaved into: Outer capsid protein P8'; Small ...
P3_RDVA Outer capsid protein P3 (Core protein P3)
P9_RDVA Minor outer capsid protein P9
RDRP_RDVA RNA-directed RNA polymerase P1 (EC 2.7.7.48) (Replicase)
VSR_RDVA Suppressor of RNA-mediated gene silencing (Non-structural protein 10) (Pns10)

9 entries

Wound tumor virus (WTV)

Select_allDeselect_all
NSP11_WTV RNA-binding protein (Non-structural protein 12) (Pns12)
NSP4_WTV Non-structural protein 4 (Pns4)
MCE_WTV Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase P5) (EC 2.7.7.50)
P7_WTV Protein P7 (60 kDa core protein)
P8_WTV Outer capsid protein P8 (Structural protein P8)
P9_WTV Minor outer capsid protein P9 (Non-structural protein 11) (Pns11)
VP7_WTV Non-structural protein PNS7
VSR_WTV Suppressor of RNA-mediated gene silencing (Non-structural protein 10) (Pns10)
VP10_WTV Non-structural protein Pns10

6 entries

Rice dwarf virus (isolate O) (RDV)

Select_allDeselect_all
NSP12_RDVO Non-structural protein 12A (Pns12A)
P8_RDVO Outer capsid protein P8 (Structural protein P8) [Cleaved into: Outer capsid protein P8'; Small ...
P7_RDVO Protein P7 (55 kDa core protein)
P2_RDVO Minor outer capsid protein P2
P9_RDVO Minor outer capsid protein P9
VSR_RDVO Suppressor of RNA-mediated gene silencing (Non-structural protein 10) (Pns10)

3 entries

Wound tumor virus (strain NJ) (WTV)

Select_allDeselect_all
NSP11_WTVNJ RNA-binding protein (Non-structural protein 12) (Pns12)
P7_WTVNJ Protein P7 (60 kDa core protein)
P9_WTVNJ Minor outer capsid protein P9 (Non-structural protein 11) (Pns11)

1 entry

Rice dwarf virus (RDV)

P9_RDV Minor outer capsid protein P9

1 entry

Rice dwarf virus (isolate S) (RDV)

P8_RDVS Outer capsid protein P8 (Structural protein P8) [Cleaved into: Outer capsid protein P8'; Small ...