Alphacarmovirus

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement proteins and capsid protein (which acts as a viral suppressor of RNA silencing ) are translated from subgenomic RNAs.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement proteins.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Matching UniProtKB/Swiss-Prot entries

7 entries grouped by strain (browse by keywords)

All proteins are shown (view only 4 complete proteome entries)

4 entries

Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

Select_allDeselect_all
CAPSD_CARMS Capsid protein (Coat protein) (p38)
MP2_CARMS Double gene block protein 2 (DGBp2) (Movement protein P9)
MP1_CARMS Double gene block protein 1 (DGBp1) (Movement protein P7)
RDRP_CARMS Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]

3 entries

Carnation mottle virus (CarMV)

Select_allDeselect_all
CAPSD_CARMV Capsid protein (Coat protein) (p38)
MP1_CARMV Double gene block protein 1 (DGBp1) (Movement protein P7)
RDRP_CARMV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]