Panicovirus

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement protein, p6.6, p15 and capsid proteins are translated from subgenomic RNAs.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Matching UniProtKB/Swiss-Prot entries

5 entries grouped by protein (browse by keywords)

1 entry

Capsid protein (Coat protein) (p26)

CAPSD_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

1 entry

Putative movement protein p6.6

MP6_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

1 entry

Probable movement protein p8

MP8_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

1 entry

Protein p15

P15_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

1 entry

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p112)

RDRP_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain