Pecluvirus

VIRION

Non-enveloped, rod shaped with helical symmetry. The virion is composed of two segments 190 and 245 nm in length, and 21 nm in diameter.

GENOME

Segmented, linear, ssRNA(+) genome. The two segments are 5.9 and 4.8 kb in size respectively. The genomic RNAs are capped.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. The RNA-1 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. Ribosomal leaky scanning allows expression of both P39 (probably required for vector transmission) and TGB3. TGB1, TGB2 and TGB3 function as movement proteins. The 15k protein is a suppressor of RNA silencing.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid protein, movement proteins. The capsid protein is directly expressed from RNA-2.
  7. Virus assembly in the cytoplasm. Each segment (if multipartite) is encapsidated.
  8. Viral movement proteins probably mediate virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

P15 of peanut clump virus suppresses RNA silencing .

Matching UniProtKB/Swiss-Prot entries

7 entries grouped by strain (browse by keywords)

7 entries

Peanut clump virus (isolate 87/TGTA2) (PCV) reference strain

Select_allDeselect_all
CAPSD_PCV87 Capsid protein (Coat protein) (P23)
P39_PCV87 Protein P39
RDRP_PCV87 Replicase large subunit (EC 2.1.1.-) (EC 2.7.7.-) (EC 2.7.7.48) (EC 3.6.4.13) (RNA-directed RNA ...
VSR_PCV87 Suppressor of RNA silencing (15kD cysteine rich protein) (P15)
TGB2_PCV87 Movement protein TGB2 (P14) (Triple gene block 2 protein) (TGBp2)
TGB1_PCV87 Movement protein TGB1 (P51) (Triple gene block 1 protein) (TGBp1)
TGB3_PCV87 Movement protein TGB3 (P17) (Triple gene block 3 protein) (TGBp3)