Goukovirus

VIRION

Enveloped, spherical. Diameter from 80 to 120nm. Glycoproteins at the surface of the envelope are arranged on an icosahedral lattice, with T=12 symmetry.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 6.4kb, M segment about 3.2kb and S segment about 1.7kb.
Encodes for six proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment. mRNAs are capped by L protein during synthesis using cap snatching , but are not polyadenylated.

REPLICATION

CYTOPLASMIC

  1. Virus attaches to host receptors though Gn-Gc glycoprotein dimer, and is endocytosed into vesicles in the host cell.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid segments are released in the cytoplasm.
  3. Transcription, viral mRNAs are capped in the cytoplasm.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. The ribonucleocapsids buds, releasing the virion.