Betacarmovirus

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Linear, ssRNA(+) genome of about 4kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement proteins and capsid protein (which acts as a viral suppressor of RNA silencing ) are translated from subgenomic RNAs.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement proteins.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Turnip crinkle virus encodes capsid protein P38 which acts as suppressor of RNA silencing .

Matching UniProtKB/Swiss-Prot entries

4 entries grouped by strain (browse by keywords)

4 entries

Turnip crinkle virus (TCV) reference strain

Select_allDeselect_all
CAPSD_TCV Capsid protein (Coat protein) (p38)
MP1_TCV Double gene block protein 1 (DGBp1) (Movement protein P8)
MP2_TCV Double gene block protein 2 (DGBp2) (Movement protein P9)
RDRP_TCV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]