Mastrevirus

VIRION

Non-enveloped, about 38 nm in length and 22 nm in diameter (for MSV), twinned (geminate) incomplete T=1 icosahedral symmetry capsid that contains 22 pentameric capsomers made of 110 capsid proteins (CP). Each geminate particle contains only a single circular ssDNA.

GENOME

Monopartite, circular, ssDNA genome (+) genome of about 2.6-2.8 kb. 3’ terminus has no poly(A) tract. There are coding regions in both the virion (positive) and complementary (negative) sense strands.
The genome is replicated through double-stranded intermediates. The replication (Rep) protein initiates and terminates rolling circle replication, the host DNA polymerase being used for DNA replication itself. There is a potential stem-loop structure in the long intergenic region (LIR) that includes a conserved nona-nucleotide sequence (TAATATTAC) where ssDNA synthesis is initiated.
The short intergenic region (SIR) contains bidirectional polyadenylation signals.

GENE EXPRESSION

Transcription is bidirectional from the long intergenic region (LIR) which contains two divergent promoters: a V-(virion) and a C-(complementary) sense. The resulting C- and V-transcripts encode four proteins. On the V-sense: the movement and CP. On the C-sense: RepA and Rep (which is expressed as a splice product of RepA and RepB ORFs).

REPLICATION

NUCLEAR

  1. Virus penetrates into the host cell.
  2. Uncoating, the viral ssDNA genome penetrates into the nucleus.
  3. The ssDNA is converted into dsDNA with the participation of cellular factors.
  4. bidirectional dsDNA transcription from the IR promoter produces viral mRNAs and translation of viral proteins.
  5. Replication is initiated by cleavage of the(+)strand by REP, and occurs by rolling circle producing ssDNA genomes.
  6. These newly synthesized ssDNA can either
    a) be converted to dsDNA and serve as a template for transcription/replication
    b) be encapsidated by capsid protein and form virions released by cell lysis
    c) be transported outside the nucleus, to a neighboring cell through plasmodesmata (cell-cell movement) with the help of viral movement proteins.

Host-virus interaction

Cell-cycle modulation

Mastrevirus protein RepA is responsible for inhibiting host retinoblastoma protein and inducing transition from the G1 to S phase in preparation for virus replication since the virus targets differentiated non-dividing cells. .

Matching UniProtKB/Swiss-Prot entries

55 entries grouped by strain (browse by keywords)

All proteins are shown (view only 52 complete proteome entries)

4 entries

Bean yellow dwarf virus (BeYDV) reference strain

Select_all Deselect_all  
CAPSD_BEYDVCapsid protein (Coat protein) (CP)
MP_BEYDVMovement protein (MP)
REPA_BEYDVReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_BEYDVReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype A (isolate South Africa) (MSV) reference strain

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CAPSD_MSVSCapsid protein (Coat protein) (CP)
MP_MSVSMovement protein (MP)
REPA_MSVSReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVSReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Miscanthus streak virus (isolate 91) (MiSV) reference strain

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CAPSD_MISV9Capsid protein (Coat protein) (CP)
MP_MISV9Putative movement protein (MP)
REPA_MISV9Replication-associated protein A (RepA) (EC 3.1.21.-)
REP_MISV9Replication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) reference strain

Select_all Deselect_all  
CAPSD_SSVNCapsid protein (Coat protein) (CP)
MP_SSVNMovement protein (MP)
REPA_SSVNReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_SSVNReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Wheat dwarf virus (isolate Sweden) (WDV) reference strain

Select_all Deselect_all  
CAPSD_WDVSCapsid protein (Coat protein) (CP)
MP_WDVSMovement protein (MP)
REPA_WDVSReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_WDVSReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype A (isolate Kenya) (MSV)

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CAPSD_MSVKCapsid protein (Coat protein) (CP)
MP_MSVKMovement protein (MP)
REPA_MSVKReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVKReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype A (isolate Nigeria) (MSV)

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CAPSD_MSVNCapsid protein (Coat protein) (CP)
MP_MSVNMovement protein (MP)
REPA_MSVNReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVNReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype B (isolate Tas) (MSV)

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CAPSD_MSVTACapsid protein (Coat protein) (CP)
MP_MSVTAMovement protein (MP)
REPA_MSVTAReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVTAReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype C (isolate Set) (MSV)

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CAPSD_MSVSECapsid protein (Coat protein) (CP)
MP_MSVSEMovement protein (MP)
REPA_MSVSEReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVSEReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype D (isolate Raw) (MSV)

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CAPSD_MSVRACapsid protein (Coat protein) (CP)
MP_MSVRAMovement protein (MP)
REPA_MSVRAReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVRAReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Maize streak virus genotype E (isolate Pat) (MSV)

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CAPSD_MSVPACapsid protein (Coat protein) (CP)
MP_MSVPAMovement protein (MP)
REPA_MSVPAReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_MSVPAReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Panicum streak virus (isolate Kenya) (PanSV)

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CAPSD_PASVKCapsid protein (Coat protein) (CP)
MP_PASVKMovement protein (MP)
REPA_PASVKReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_PASVKReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

4 entries

Tobacco yellow dwarf virus (strain Australia) (TYDV)

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CAPSD_TYDVACapsid protein (Coat protein) (CP)
MP_TYDVAMovement protein (MP)
REPA_TYDVAReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_TYDVAReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)

3 entries

Chloris striate mosaic virus (CSMV)

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CAPSD_CSMVCapsid protein (Coat protein) (CP)
REPA_CSMVReplication-associated protein A (RepA) (EC 3.1.21.-)
REP_CSMVReplication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-)