Microviridae

VIRION

Non-enveloped, round, T=1 icosahedral symmetry, about 30 nm in diameter. The capsid consists of 12 pentagonal trumpet-shaped pentomers. The virion is composed of 60 copies each of the F, G, and J proteins, and 12 copies of the H protein. There are 12 spikes which are each composed of 5 G and one H proteins.


GENOME

Circular, ssDNA(+) genome of 4.4 to 6.1kb. replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Early and late genes promoters tightly regulate the timing of gene expression, which is crucial for the replication cycle.

REPLICATION

CYTOPLASMIC

  1. Pilus-mediated adsorption of the virus onto host cell
  2. The proteins of the capsid perform Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA I (RF-I).
  4. Early viral genes are transcribed by host RNA polymerase, producing viral replication proteins.
  5. Viral protein A cleaves RF-I(+) DNA strand at the origin of replication and covalently attaches itself to the DNA.
  6. (+)strand replication occurs by rolling circle, which is converted to dsDNA by host polymerase, generating RF-II molecules (amplification of RF-I).
  7. Late viral genes are transcribed by host RNA polymerase.
  8. Procapsid assembly in the cytoplasm.
  9. Viral protein C binds to replication complex, inducing synthesis and packaging of neo-synthesized (+)ssDNA genomes (RF-III) into procapsids.
  10. Procapsids maturation occurs in host cytoplasm
  11. Viral lysozyme attacks the peptidoglycan wall, inducing cell lysis and releasing mature virions.

Matching UniProtKB/Swiss-Prot entries

78 entries grouped by strain (browse by keywords)

11 entries

Bdellovibrio phage phiMH2K (Bacteriophage phiMH2K) reference strain

Select_all Deselect_all  
B_BPPHMInternal scaffolding protein VP3
C_BPPHMProtein VP5
F_BPPHMCapsid protein VP1 (VP1)
H_BPPHMMinor spike protein H (H protein) (Pilot protein) (Protein VP2) (VP2)
J_BPPHMDNA binding protein VP8
N_BPPHMUncharacterized protein N
REP_BPPHMReplication-associated protein VP4 (Rep) (VP4) (EC 3.1.21.-) (EC 6.5.1.1)
W_BPPHMUncharacterized protein W
X_BPPHMUncharacterized protein X
Y_BPPHMUncharacterized protein Y
Z_BPPHMUncharacterized protein Z

10 entries

Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) reference strain

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B_BPPHSInternal scaffolding protein B (GPB)
C_BPPHSProtein C
D_BPPHSExternal scaffolding protein D (GPD)
E_BPPHSLysis protein E (Protein E) (GPE)
F_BPPHSCapsid protein F (F protein) (GPF)
G_BPPHSMajor spike protein G (G protein) (GPG)
H_BPPHSMinor spike protein H (H protein) (Pilot protein)
J_BPPHSDNA-binding protein J (Small core protein)
K_BPPHSProtein K
REPA_BPPHSReplication-associated protein A (EC 3.1.21.-) (EC 6.5.1.1) (RepA)

9 entries

Spiroplasma virus 4 (SpV4) reference strain

Select_all Deselect_all  
B_SPV4Internal scaffolding protein ORF3
C_SPV4Protein ORF5
F_SPV4Capsid protein VP1 (VP1)
H_SPV4Minor spike protein H (H protein) (Pilot protein)
J_SPV4DNA binding protein ORF8
ORF6_SPV4Uncharacterized protein ORF6
ORF7_SPV4Uncharacterized protein ORF7
ORF9_SPV4Uncharacterized protein ORF9
REP_SPV4Replication-associated protein ORF2 (EC 3.1.21.-) (EC 6.5.1.1) (Rep)

8 entries

Chlamydia phage 1 (Bacteriophage Chp1) reference strain

Select_all Deselect_all  
B_BPCHPInternal scaffolding protein VP3 (Protein VP3) (VP3)
C_BPCHPProtein ORF5
F_BPCHPCapsid protein VP1 (Protein VP1) (VP1)
H_BPCHPMinor spike protein H (H protein) (Pilot protein) (Protein VP2) (VP2)
J_BPCHPDNA binding protein ORF8
ORF6_BPCHPUncharacterized protein ORF6
ORF7_BPCHPUncharacterized protein ORF7
REP_BPCHPReplication-associated protein ORF4 (EC 3.1.21.-) (EC 6.5.1.1) (Rep)

10 entries

Enterobacteria phage G4 (Bacteriophage G4)

Select_all Deselect_all  
G_BPG4Major spike protein G (G protein) (GPG)
VGA_BPG4A protein (GPA)
VGB_BPG4Scaffolding protein B (GPB)
VGC_BPG4C protein
VGD_BPG4Scaffolding protein D (GPD)
VGE_BPG4Lysis protein (E protein) (GPE)
VGF_BPG4Capsid protein (F protein) (GPF)
VGH_BPG4Minor spike protein (H protein) (Pilot protein)
VGJ_BPG4Small core protein (J protein)
VGK_BPG4K protein

10 entries

Enterobacteria phage S13 (Bacteriophage S13)

Select_all Deselect_all  
G_BPS13Major spike protein G (G protein) (GPG)
VGA_BPS13A' protein (GPA)
VGB_BPS13Scaffolding protein B (GPB)
VGC_BPS13C protein
VGD_BPS13Scaffolding protein D (GPD)
VGE_BPS13Lysis protein (E protein) (GPE)
VGF_BPS13Capsid protein (F protein) (GPF)
VGH_BPS13Minor spike protein (H protein) (Pilot protein)
VGJ_BPS13Small core protein (J protein)
VGK_BPS13K protein

10 entries

Enterobacteria phage alpha3 (Bacteriophage alpha-3)

Select_all Deselect_all  
G_BPAL3Major spike protein G (G protein) (GPG)
VGA_BPAL3A protein (GPA)
VGB_BPAL3Scaffolding protein B (GPB)
VGC_BPAL3C protein
VGD_BPAL3Scaffolding protein D (GPD)
VGE_BPAL3Lysis protein (E protein) (GPE)
VGF_BPAL3Capsid protein (F protein) (GPF)
VGH_BPAL3Minor spike protein (H protein) (Pilot protein)
VGJ_BPAL3Small core protein (J protein)
VGK_BPAL3K protein

10 entries

Enterobacteria phage phiK (Bacteriophage phi-K)

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G_BPPHKMajor spike protein G (G protein) (GPG)
VGA_BPPHKA protein (GPA)
VGB_BPPHKScaffolding protein B (GPB)
VGC_BPPHKC protein
VGD_BPPHKScaffolding protein D (GPD)
VGE_BPPHKLysis protein (E protein) (GPE)
VGF_BPPHKCapsid protein (F protein) (GPF)
VGH_BPPHKMinor spike protein (H protein) (Pilot protein)
VGJ_BPPHKSmall core protein (J protein)
VGK_BPPHKK protein