Molecular biology
VIRION
Nonenveloped, head-tail structure. The head is about 80 nm in diameter. Virions have 3 subterminal kinked fibers and a conical tip with a single long fiber.
GENOME
Linear, dsDNA genome of about 121 kb, containing about 160 genes.
GENE EXPRESSION
REPLICATION
CYTOPLASMIC
- Adsorption: the phage attaches to target cell trhough its tail fibers.
- Viral lysozyme degrades cell wall locally
- Injection: viral DNA is injected in host cell periplasm
- DNA genome is circularized or integrated into host chromosome
- Transcription and traduction of early genes
- Replication of genomic DNA
- Transcription and traduction of late genes
- Assembly of new virions in host cell cytoplasm
- Mature virions are released from the cell by lysis