Leishmaniavirus

VIRION

Non enveloped, icosahedral virion composed of a single capsid protein (CP), about 33 nm in diameter. The capsid has a T=2 icosahedral symmetry.

GENOME

Linear dsRNA genome of 5.3 kb. The genome has two large, overlapping ORFs on the positive strand respectively encoding CP and RdRp, and a small 5’-proximal potential ORF.

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a mRNA, which is translocated to the cell cytoplasm where it is translated.
Translation is initiated on a unique internal ribosome entry site (IRES) element situated at the 5’-UTR.
L-A virus seems to furnish its transcript with a cap structure derived from host mRNAs by cap snatching . m(7)Gp from the cap donor is transferred to the 5’ end of the viral transcript, thus preserving the 5’ α-and β-phosphates of the transcript in the triphosphate linkage of the final product. The plus-strand viral transcript directs the translation of the major CP (Gag) and the minor fusion protein CP-RdRP (Gag-Pol) via a -1 ribosomal frameshift.

REPLICATION

CYTOPLASMIC

  1. Virus remains intracellular.
  2. Transcription of the dsRNA genome by viral polymerase occurs inside the virion, so that dsRNA is never exposed to the cytoplasm. This plus-strand transcript is used as template for translation.
  3. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  4. Mature virions may be transmitted to new cells during cell division.

Matching UniProtKB/Swiss-Prot entries

3 entries grouped by strain (browse by keywords)

3 entries

Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) reference strain

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CAPSD_LRVLGMajor capsid protein (Major coat protein)
ORF1_LRVLGPutative ORF1 protein
RDRP_LRVLGProbable RNA-directed RNA polymerase (EC 2.7.7.48)