Molecular biology
VIRION
Non enveloped, icosahedral, non-turreted virion with a triple capsid structure, about 90-95 nm in diameter. The intermediate capsid has an icosahedral T=13 symmetry, the inner capsid an icosahedral symmetry T=2.
GENOME
Segmented dsRNA linear genome. Contains 11 segments encoding for at least 11 proteins. Segment 1 of MpRV is 5.8 kb long, the longest segment of any of the characterized reoviruses.
GENE EXPRESSION
The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response of dsRNA. The viral polymerase synthesizes a capped mRNA from each dsRNA segment. This capped mRNA is translocated to the cell cytoplasm where it is translated.
REPLICATION
CYTOPLASMIC
- Virus penetrates into the cytoplasm.
- Transcription of the dsRNA genome by viral polymerase. This plus-strand transcript is used as template for translation.
- (+)RNAs are encapsidated in virion particle, inside which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
- Mature virions are released.