Minichromosome synthesis (rcDNA repair)

From rcDNA to cccDNA

The formation of the cccDNA from the rcDNA is incompletely understood, but several steps are essential for the synthesis:

- The removal of the polymerase covalently attached to the 5' end of the negative strand.

- The removal of the primer from the 5' plus strand.

- The removal of the short redundancy region r from the minus strand.

- Fill in gaps in the positive strand

- Fill in gaps at the ends of both strands which must be ligated to yield closed circles (Nassal 2008 ) Rating=Review

This page has been funded in part by Hoffmann-La Roche