Parapoxvirus

VIRION

Enveloped, ovoid virion, 220-300nm long and 140-170nm wide. The surface membrane displays surface filaments. Two distinct infectious virus particles exists: the intracellular mature virus (IMV) and the extracellular enveloped virus (EEV).

GENOME

Linear, dsDNA genome of 130-150kb. The linear genome is flanked by inverted terminal repeat (ITR) sequences which are covalently-closed at their extremities.
Bovine papular stomatitis virus strain BV-AR02 genome at Poxvirus Bioinformatics Ressource center

GENE EXPRESSION

REPLICATION

CYTOPLASMIC

  1. Attachement of the viral proteins to host glycosaminoglycans (GAGs) mediates endocytosis of the virus into the host cell.
  2. Fusion with the plasma membrane to release the core into the host cytoplasm.
  3. Early phase: early genes are transcribed in the cytoplasm by viral RNA polymerase. Early expression begins at 30 minutes post-infection.
  4. Core is completely uncoated as early expression ends, viral genome is now free in the cytoplasm.
  5. Intermediate phase: Intermediate genes are expressed, triggering genomic DNA replication at approximately 100 minutes post-infection.
  6. Late phase: Late genes are expressed from 140 min to 48 hours post-infection, producing all structural proteins.
  7. Assembly of progeny virions starts in cytoplasmic viral factories, producing an spherical immature particle. This virus particle matures into brick-shaped intracellular mature virion (IMV).
  8. IMV virion can be released upon cell lysis, or can acquire a second double membrane from trans-Golgi and bud as external enveloped virion (EEV).

Host-virus interaction

Apoptosis modulation

The orf virus encodes a viral bcl-2 protein (v-Bcl2) that prevents host apoptosis .

NF-kappa-B modulation

Orf virus ORFV121 physically interacts with NF-kappaB-p65 in the cell cytoplasm and thereby inhibits host NF-kappaB signaling pathway .

Cell cycle modulation

The poxvirus anaphase promoting complex regulator (PACR) promotes viral replication by manipulating the anaphase promoting complex/cyclosome (APC/C), thus promoting the host differentiated G0 cells to enter G1 .

Matching UniProtKB/Swiss-Prot entries

7 entries grouped by protein (browse by keywords)

All proteins are shown (view only 1 complete proteome entries)

1 entry

DNA polymerase (EC 2.7.7.7)

DPOL_ORFN2
Orf virus (strain NZ2) (OV NZ-2)

1 entry

Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP pyrophosphatase)

DUT_ORFN2
Orf virus (strain NZ2) (OV NZ-2)

1 entry

Poly(A) polymerase catalytic subunit (EC 2.7.7.19) (Poly(A) polymerase large subunit) (PAP-L)

PAP1_ORFN2
Orf virus (strain NZ2) (OV NZ-2)

1 entry

Protein ORFV073

V073_ORFSA
Orf virus (strain Goat/Texas/SA00/2000) (OV-SA00) (Orf virus-San Angelo 2000) reference strain

2 entries

Vascular endothelial growth factor homolog

Select_allDeselect_all
VEGFH_ORFN2
Orf virus (strain NZ2) (OV NZ-2)
VEGFH_ORFN7
Orf virus (strain NZ7) (OV NZ-7)

1 entry

10 kDa fusion protein

VFUS_ORFN2
Orf virus (strain NZ2) (OV NZ-2)