Molluscipoxvirus

VIRION

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Enveloped, brick-shaped about 320x250x200nm. Two distinct infectious virus particles exists: the intracellular mature virus (IMV) and the extracellular enveloped virus (EEV).

GENOME

Linear, dsDNA genome about 190kb. The linear genome is flanked by inverted terminal repeat (ITR) sequences which are covalently-closed at their extremities.
Molluscum contagiosum virus strain subtype 1 genome at Poxvirus Bioinformatics Ressource center

GENE EXPRESSION

REPLICATION

CYTOPLASMIC

  1. Attachement of the viral proteins to host glycosaminoglycans (GAGs) mediates endocytosis of the virus into the host cell.
  2. Fusion with the plasma membrane to release the core into the host cytoplasm.
  3. Early phase: early genes are transcribed in the cytoplasm by viral RNA polymerase. Early expression begins at 30 minutes post-infection.
  4. Core is completely uncoated as early expression ends, viral genome is now free in the cytoplasm.
  5. Intermediate phase: Intermediate genes are expressed, triggering genomic DNA replication at approximately 100 minutes post-infection.
  6. Late phase: Late genes are expressed from 140 min to 48 hours post-infection, producing all structural proteins.
  7. Assembly of progeny virions starts in cytoplasmic viral factories, producing an spherical immature particle. This virus particle matures into brick-shaped intracellular mature virion (IMV).
  8. IMV virion can be released upon cell lysis, or can acquire a second double membrane from trans-Golgi and bud as external enveloped virion (EEV).

Host-virus interaction

Apoptosis modulation

Molluscipoxviruses encode a viral FLICE-inhibitory protein (v-FLIP) that prevents apoptosis induced by death receptors .

NF-kappa-B modulation

MC159 protein from the molluscum contagiosum inhibits NF-kappa-B activation by interacting with the IkB kinase complex .