HIV-1 GAG-POL precursor (pr160GagPol)
FUNCTION
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Mediates (with pr55Gag) the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi) .
Finally, the release of the protease from pr160GagPol triggers the virion maturation .
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INTERACTIONS
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Recognizes the packaging sequence (Psi) and allows gRNA dimerization and packaging. Also binds the Bro1 domain of Alix, STAU1, ABCE1, DHX9, EAP30 and interacts with the plasma membrane during the process of virion assembly. Interacts with the plasma through the phosphatidyl-inositol-4,5-bisphosphate binding domain and the myristoyl moiety. Interacts with Env gp41 C-terminus. Interacts with host AP-2 and AP-3 targeting the polyprotein to multivesicular bodies.
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SUBCELLULAR LOCATION
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- Late endosomes/Multivesicular bodies
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TIMING OF EXPRESSION
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Wrapping up the bad news: HIV assembly and release
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The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions
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Aspects of HIV-1 assembly that promote primer tRNA(Lys3) annealing to viral RNA
Jenan Saadatmand, Lawrence Kleiman
Virus Res. November 2012; 169: 340-348
Role of the HIV-1 Matrix Protein in Gag Intracellular Trafficking and Targeting to the Plasma Membrane for Virus Assembly
Ruba H. Ghanam, Alexandra B. Samal, Timothy F. Fernandez, Jamil S. Saad
Front Microbiol 2012; 3: 55
Properties and functions of the nucleocapsid protein in virus assembly
Delphine Muriaux, Jean-Luc Darlix
RNA Biol December 2010; 7: 744-753
Specific recognition of the HIV-1 genomic RNA by the Gag precursor
Ekram W. Abd El-Wahab, Redmond P. Smyth, Elodie Mailler, Serena Bernacchi, Val?rie Vivet-Boudou, Marcel Hijnen, Fabrice Jossinet, Johnson Mak, Jean-Christophe Paillart, Roland Marquet
Nat Commun 2014; 5: 4304
Retrospective on the all-in-one retroviral nucleocapsid protein
Jean-Luc Darlix, Hugues de Rocquigny, Olivier Mauffret, Yves M?ly
Virus Res. June 4, 2014;
HIV-1 assembly, budding, and maturation
Wesley I. Sundquist, Hans-Georg Kr?usslich
Cold Spring Harb Perspect Med July 2012; 2: a006924
HIV-1 assembly, budding, and maturation
Wesley I. Sundquist, Hans-Georg Kr?usslich
Cold Spring Harb Perspect Med July 2012; 2: a006924
Autoprocessing of HIV-1 protease is tightly coupled to protein folding
J. M. Louis, G. M. Clore, A. M. Gronenborn
Nat. Struct. Biol. September 1999; 6: 868-875