Polyuridine endonuclease
. If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails, activate host dsRNA sensors and trigger cell antiviral response. This activity is only found in vertebrate Nidovirus.
Enzymatic reaction:
- Polyuridine endonuclease RHEA:67732
- 2'-O transesterification generates a 2'3'-cyclic phosphate (2'3'-cP) RHEA:67792 EC=4.6.1.-
- RNA 2',3'-cyclic 3'-phosphodiesterase RHEA:11828 EC 3.1.4.58
Domain: PF19215
Mechanism
Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
Monica C Pillon, Meredith N Frazier, Lucas B Dillard, Jason G Williams, Seda Kocaman, Juno M Krahn, Lalith Perera, Cassandra K Hayne Jacob Gordon, Zachary D Stewart, Mack Sobhany, Leesa J Deterding, Allen L Hsu, Venkata P Dandey, Mario J Borgnia, Robin E Stanley
Nat Commun. 2021 Jan 27;12(1):636
Crystal structure and mechanistic determinants of SARS coronavirus nonstructural protein 15 define an endoribonuclease family
Stefano Ricagno, Marie-Pierre Egloff, Rachel Ulferts, Bruno Coutard, Didier Nurizzo, Val?rie Campanacci, Christian Cambillau, John Ziebuhr, Bruno Canard
Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11892-7