Orthotulasvirus (taxid:2948852)

VIRION

Unknown, discovered using viral metagenomics. Presumably non enveloped.

GENOME

Negative-stranded RNA linear genome, about 12 kb in size. Encodes for three proteins.

Enzymes: RNA dependent RNA polymerase, endonuclease? (Cap snatching)

GENE EXPRESSION

The genome is ambisens. The viral RNA dependent RNA polymerase (L) binds to a promoter and transcribes a messenger RNA. Transcription is terminated by a strong hairpin sequence at the end of each gene. mRNAs are capped, probably by L protein during synthesis.

ENZYMES

• RNA-dependent RNA polymerase [L]
• Cap Snatching endonuclease [L]

REPLICATION

CYTOPLASMIC

1. The ribonucleocapsids enters a cell by Fungal intercellular connetions
2. Transcription, viral mRNAs are capped in the cytoplasm.
3. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
4. The ribonucleocapsids is transmitted to another cell by Fungal intercellular connetions