Vaccinivirus (taxid:2733293)

VIRION

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Non enveloped, large (50 nm diameter) icosahedral particles composed of 420 subunits with a T=7 symmetry . It has not been described whether handedness of capsid is laevo or dextro, here the pictures displays a laevo form.

GENOME

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Monopartite, open circular, double stranded DNA of about 9.8kb with discontinuities in both strands: one in the transcribed strand and one to three in the non-transcribed strand. Codes for 7 proteins.

GENE EXPRESSION

The genomic RNA and its spliced derivatives serves as polycistronic mRNA for viral proteins. Polycistronic translation may depends on ORF4(Tav) which is would be a translation reinitiation factor.

ENZYMES

REPLICATION

CYTOPLASMIC / NUCLEAR

  1. Attachment of viral proteins to host receptors mediates entry into the host cell.
  2. The viral dsDNA is released into the nucleus.
  3. Transcribed by host RNA polymerase II.
  4. mRNA translation produces viral proteins.
  5. Genomic RNA is retrotranscribed into new dsDNA genomes in the cytoplasm.
  6. Genomes are encapsidated by the capsid protein and form new virions.
  7. The virion infects either a new cell by plasmodesmata movement, or by insect vector uptake.