Ebolavirus

Molecular biology

Filamentous 970 nm long for Ebolavirus. Diameter is about 80nm.

GENOME

Negative-stranded RNA linear genome, about 18-19 kb in size. Encodes for seven proteins.

GENE EXPRESSION

The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis.
The primary product of the unedited transcript of GP gene yields a smaller non-structural glycoprotein sGP which is efficiently secreted from infected cells. RNA editing allows expression of full-length GP.

REPLICATION

CYTOPLASMIC

  1. Attachment to host receptors through GP glycoprotein like DC-SIGN and DC-SIGNR
  2. Cellular receptor like HAVCR1 phosphatidyl serine on virion membrane and a signal is transduced into the cell that trigger the macropinocytosis program by apoptotic mimicry.
  3. The virion enters the cell by Macropinocytosis . In some culture cells, GP glycoprotein can be processed by host Cathepsin L and Cathepsin B into 19kDa GP1 . But this processing is not happening in all cells or for all ebolavirus .
  4. GP1 interacts with host NPC1, in late macropinosome and promotes Fusion of virus membrane with the vesicle membrane . The ribonucleocapsid is then released into the cytoplasm.
  5. Sequential transcription, viral mRNAs are capped and polyadenylated by polymerase stuttering in the cytoplasm.
  6. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  7. The ribonucleocapsid interacts with the matrix protein, and buds via the host ESCRT complexes from the plasma membrane , releasing the virion .

Host-virus interaction

Inhibition of host Interferon induction

Vp35 protein prevents host interferon induction by Inhibition of host IKBKE by virus

Inhibition of host interferon signaling

Vp24 protein block interferon signaling by preventing STAT1 signaling. May act by binding host karyopherin ? proteins, thereby preventing them from transporting the tyrosine-phosphorylated transcription factor STAT1 to the nucleus. May directly bind to STAT1 to prevent its signaling .

Antigenic subvertion

Small Glycoprotein (sGP) is a secreted truncated version of GP that prevent host antibodies anti-GP to effectively neutralize it .

Inhibition of acquired immune response

Ebolavirus may encode for a superantigen, thereby inhibiting Vbeta t cells

Matching UniProtKB/Swiss-Prot entries

62 entries grouped by strain (browse by keywords)

All proteins are shown (view only 45 complete proteome entries)

9 entries

Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) reference strain

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NCAP_EBORR Nucleoprotein (Nucleocapsid protein) (Protein N)
L_EBORR RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) ...
VGP_EBORR Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VP40_EBORR Matrix protein VP40 (Membrane-associated protein VP40)
VP24_EBORR Membrane-associated protein VP24
VP35_EBORR Polymerase cofactor VP35
VSGP_EBORR Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...
VSSGP_EBORR Super small secreted glycoprotein (SsGP)
VP30_EBORR Minor nucleoprotein VP30 (Transcription activator VP30)

9 entries

Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) reference strain

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NCAP_EBOZM Nucleoprotein (Nucleocapsid protein) (Protein N)
L_EBOZM RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) ...
VGP_EBOZM Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VP24_EBOZM Membrane-associated protein VP24
VP40_EBOZM Matrix protein VP40 (Membrane-associated protein VP40)
VP35_EBOZM Polymerase cofactor VP35
VP30_EBOZM Minor nucleoprotein VP30 (Transcription activator VP30)
VSGP_EBOZM Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...
VSSGP_EBOZM Super small secreted glycoprotein (SsGP)

9 entries

Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)

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NCAP_EBORE Nucleoprotein (Nucleocapsid protein) (Protein N)
L_EBORE RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) ...
VGP_EBORE Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VP24_EBORE Membrane-associated protein VP24
VP35_EBORE Polymerase cofactor VP35
VSSGP_EBORE Super small secreted glycoprotein (SsGP)
VP30_EBORE Minor nucleoprotein VP30 (Transcription activator VP30)
VP40_EBORE Matrix protein VP40 (Membrane-associated protein VP40)
VSGP_EBORE Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...

9 entries

Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)

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L_EBOSU RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) ...
NCAP_EBOSU Nucleoprotein (Nucleocapsid protein) (Protein N)
VGP_EBOSU Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VSGP_EBOSU Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...
VP35_EBOSU Polymerase cofactor VP35
VP30_EBOSU Minor nucleoprotein VP30 (Transcription activator VP30)
VSSGP_EBOSU Super small secreted glycoprotein (SsGP)
VP40_EBOSU Matrix protein VP40 (Membrane-associated protein VP40)
VP24_EBOSU Membrane-associated protein VP24

9 entries

Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

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NCAP_EBOZ5 Nucleoprotein (Nucleocapsid protein) (Protein N)
L_EBOZ5 RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) ...
VP30_EBOZ5 Minor nucleoprotein VP30 (Transcription activator VP30)
VP35_EBOZ5 Polymerase cofactor VP35
VGP_EBOZ5 Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VP24_EBOZ5 Membrane-associated protein VP24
VP40_EBOZ5 Matrix protein VP40 (Membrane-associated protein VP40)
VSSGP_EBOZ5 Super small secreted glycoprotein (SsGP)
VSGP_EBOZ5 Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...

5 entries

Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)

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NCAP_EBOG4 Nucleoprotein (Nucleocapsid protein) (Protein N)
VGP_EBOG4 Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VP40_EBOG4 Matrix protein VP40 (Membrane-associated protein VP40)
VSGP_EBOG4 Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...
VP24_EBOG4 Membrane-associated protein VP24

3 entries

Sudan ebolavirus (strain Boniface-76) (SEBOV) (Sudan Ebola virus)

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NCAP_EBOSB Nucleoprotein (Nucleocapsid protein) (Protein N)
VGP_EBOSB Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VSGP_EBOSB Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...

3 entries

Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)

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L_EBOSM RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) ...
VGP_EBOSM Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VSGP_EBOSM Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...

2 entries

Reston ebolavirus (strain Siena/Philippine-92) (REBOV) (Reston Ebola virus)

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VGP_EBORS Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VSGP_EBORS Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...

2 entries

Tai Forest ebolavirus (strain Cote d'Ivoire-94) (TAFV) (Cote d'Ivoire Ebola virus)

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VGP_TAFVC Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VSGP_TAFVC Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...

2 entries

Zaire ebolavirus (strain Eckron-76) (ZEBOV) (Zaire Ebola virus)

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VGP_EBOEC Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
VSGP_EBOEC Pre-small/secreted glycoprotein (pre-sGP) [Cleaved into: Small/secreted glycoprotein (sGP); ...