Nodaviridae (taxid:12283)

VIRION

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Non enveloped virion, about 30 nm in diameter with a T=3 icosahedral symmetry (180 protein subunits).
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GENOME

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Segmented, bipartite linear, ssRNA(+) genome composed of RNA1=3.1 kb and RNA2=1.4 kb. Each genome segment 5' end is capped. The 3'end has no poly (A) tract.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. RNA1 encodes directly the RNA-dependent RNA polymerase (protein A), and RNA2, the capsid protein. A subgenomic RNA (sgRNA3), 3' co-linear to RNA1 is also produced and encode proteins B1 and B2.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA-1 is translated to produce the RdRp protein.
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Expression of the subgenomic RNA-3.
  7. Translation of RNA-2 encoding capsid protein alpha.
  8. Virus assembly in the cytoplasm around genomic RNA1 and RNA2.
  9. Assembled capsid protein alpha is cleaved into capsid protein beta and gamma.
  10. Release of infectious particles (lysis?).

Host-virus interaction

Suppression of RNA silencing

Nodamuravirus protein B2 and Flock house virus protein B2 act as suppressors of RNA silencing .

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

23 entries grouped by protein

1 entry

Protein B

B_SJNNV
Striped jack nervous necrosis virus (SjNNV) reference strain

2 entries

Protein B1

Select_allDeselect_all
B1_BBV
Black beetle virus (BBV)
B1_FHV
Flock house virus (FHV)

4 entries

Protein B2

Select_allDeselect_all
B2_NODAM
Nodamura virus (strain Mag115) (NoV) reference strain
B2_BBV
Black beetle virus (BBV)
B2_BOOLV
Boolarra virus (BoV)
B2_FHV
Flock house virus (FHV)

6 entries

Capsid protein alpha (EC 3.4.23.44)

Select_allDeselect_all
CAPSD_NODAM
Nodamura virus (strain Mag115) (NoV) reference strain
CAPSD_SJNNV
Striped jack nervous necrosis virus (SjNNV) reference strain

Capsid protein alpha

CAPSD_BBV
Black beetle virus (BBV)
CAPSD_BOOLV
Boolarra virus (BoV)
CAPSD_FHV
Flock house virus (FHV)
CAPSD_PAV
Pariacoto virus (PaV)

10 entries

RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase) (Protein A)

Select_allDeselect_all
RDRP_NODAM
Nodamura virus (strain Mag115) (NoV) reference strain
RDRP_SJNNV
Striped jack nervous necrosis virus (SjNNV) reference strain
RDRP_AHNNV
Atlantic halibut nervous necrosis virus (AhNNV)
RDRP_BBV
Black beetle virus (BBV)
RDRP_BOOLV
Boolarra virus (BoV)
RDRP_FHV
Flock house virus (FHV)
RDRP_GGNNV
Greasy grouper nervous necrosis virus (GGNNV) (Epinephelus tauvina nervous necrosis virus)
RDRP_MRNV
Macrobrachium rosenbergii nodavirus (MrNV)

RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase)

RDRP_PAV
Pariacoto virus (PaV)
RDRP_PRV
Pieris rapae virus

RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase)