Enveloped. extracellular baculovirus virions can be found in two forms: OV (occluded virus) and BV (budded virus). The nucleocapsid is about 21 nm x 260 nm.
Circular dsDNA, 80-180 kb in length, encoding for 100 to 180 proteins.
GENE EXPRESSION The GP64 protein is expressed by leaky scanning after a uORF.
- Attachement of the viral glycoproteins to host receptors mediates endocytosis of the virus into the host cell.
- Fusion with the plasma membrane.
- The DNA genome is released into the host nucleus.
- Immediate early phase: host RNA polymerase transcribes viral genes involved in the regulation of the replication cascade, prevention of host responses and viral DNA synthesis.
- Late phase: The virally encoded RNA polymerase expresses late genes.
- Replication of the genome by rolling circle in nuclear viral factories.
- Nucleocapsids are formed which can either bud out through the cellular membrane and disseminate the infection or be occluded for horizontal transmission.
- Occlusion phase: the virus becomes occluded in the protein polyhedrin and the polyhedral envelope (calyx) is produced. Lysis of the cell releases the occluded virus.