Alphanodavirus

VIRION

Non enveloped virion, about 30 nm in diameter with a T=3 icosahedral symmetry (180 protein subunits). RNA1 and RNA2 are copackaged into the same virion and both are required for infectivity.

GENOME

Segmented, bipartite linear ssRNA(+) genome composed of RNA1=3.1 kb and RNA2=1.4 kb. Each genome segment 5' end is capped. The 3’end has no poly (A) tract.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. RNA1 encodes directly the RNA-dependent RNA polymerase (protein A), and RNA2, the capsid protein. A subgenomic RNA (sgRNA3), 3' co-linear to RNA1 is also produced and encode proteins B1 and B2.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA-1 is translated to produce the RdRp protein.
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Expression of the subgenomic RNA-3.
  7. Translation of RNA-2 encoding capsid protein alpha.
  8. Virus assembly in the cytoplasm around genomic RNA1 and RNA2.
  9. Assembled capsid protein alpha is cleaved into capsid protein beta and gamma.
  10. Release of infectious particles (lysis?).

Host-virus interaction

Suppression of RNA silencing

Nodamuravirus protein B2 and Flock house virus protein B2 act as suppressors of RNA silencing .

Matching UniProtKB/Swiss-Prot entries

16 entries grouped by strain (browse by keywords)

All proteins are shown (view only 15 complete proteome entries)

3 entries

Nodamura virus (strain Mag115) (NoV) reference strain

Select_allDeselect_all
B2_NODAM Protein B2
CAPSD_NODAM Capsid protein alpha [Cleaved into: Capsid protein beta (EC 3.4.23.44) (Coat protein beta) ...
RDRP_NODAM RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase) (Protein A)

4 entries

Black beetle virus (BBV)

Select_allDeselect_all
B1_BBV Protein B1
B2_BBV Protein B2
CAPSD_BBV Capsid protein alpha [Cleaved into: Capsid protein beta (EC 3.4.23.44) (Coat protein beta) ...
RDRP_BBV RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase) (Protein A)

4 entries

Flock house virus (FHV)

Select_allDeselect_all
B2_FHV Protein B2
B1_FHV Protein B1
CAPSD_FHV Capsid protein alpha [Cleaved into: Capsid protein beta (EC 3.4.23.44) (Coat protein beta) ...
RDRP_FHV RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase) (Protein A)

3 entries

Boolarra virus (BoV)

Select_allDeselect_all
B2_BOOLV Protein B2
CAPSD_BOOLV Capsid protein alpha [Cleaved into: Capsid protein beta (EC 3.4.23.44) (Coat protein beta) ...
RDRP_BOOLV RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase) (Protein A)

2 entries

Pariacoto virus (PaV)

Select_allDeselect_all
CAPSD_PAV Capsid protein alpha [Cleaved into: Capsid protein beta (EC 3.4.23.44) (Coat protein beta) ...
RDRP_PAV RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (RNA replicase) (Protein A)